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AuthorZupin, Luisa
AuthorFontana, Francesco
AuthorClemente, Libera
AuthorRuscio, Maurizio
AuthorCrovella, Sergio
Available date2022-04-26T07:28:52Z
Publication Date2022-04-21
Publication NameBrazilian Journal of Microbiology
Identifierhttp://dx.doi.org/10.1007/s42770-022-00758-6
CitationZupin, L., Fontana, F., Clemente, L. et al. Comparison between nucleic acid amplification tests, antigen immunofluorescence assay, and in vitro infectivity in SARS-CoV-2 diagnosis. Braz J Microbiol (2022). https://doi.org/10.1007/s42770-022-00758-6
ISSN1517-8382
URIhttp://hdl.handle.net/10576/30432
AbstractThe number of SARS-CoV-2 detection tests requested to the laboratories has dramatically increased together with an urgent need to release reliable responses in a very short time. The two options taken into consideration and analyzed in the current study were the point-of-care test (POCT) based on the nucleic acid amplification test (NAAT) and the Antigen (Ag) rapid test. The POCT-NAAT-based assay was compared with a rapid antigen test of nasopharyngeal swab samples. If the specimen tested positive, it was followed by viral load quantification and by the functional assessment of the residual infectivity. When the initial cycle threshold (Ct) was below 20 (100%), and in the range of 20-25 (92%) and of 25-30 (88%), a great concordance between the POCT-NAAT and the Ag test was observed. Moreover, the positivity of the antigen test was well correlated to a successful infection in vitro (78%), with greater concordance when the initial Ct below 20 or above 35 (100%) and in the range 20-25 (83%). Our findings showed that most of the swabs which tested positive using the antigen test were able to infect the cells in vitro, suggesting that probably only these samples hold residual infectivity and therefore an increased risk of virus transmission at the moment of being tested.
SponsorThis work was supported by IRCCS Burlo Garofolo /Italian Ministry of Health (RC 15/2017, 03/2020, RC 47/2020).
Languageen
PublisherSpringer
SubjectAntigen test
Nucleic acid amplification test
SARS-CoV-2
TitleComparison between nucleic acid amplification tests, antigen immunofluorescence assay, and in vitro infectivity in SARS-CoV-2 diagnosis.
TypeArticle
ESSN1678-4405
dc.accessType Full Text


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