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المؤلفGamasaee N.A.
المؤلفMuhammad H.A.
المؤلفTadayon E.
المؤلفAle-Ebrahim M.
المؤلفMirpour M.
المؤلفSharifi M.
المؤلفSalihi A.
المؤلفShekha M.S.
المؤلفAlasady A.A.B.
المؤلفAziz F.M.
المؤلفAkhtari K.
المؤلفHasan, Anwarul
المؤلفFalahati M.
تاريخ الإتاحة2022-05-21T10:18:30Z
تاريخ النشر2020
اسم المنشورJournal of Biomolecular Structure and Dynamics
المصدرScopus
المعرّفhttp://dx.doi.org/10.1080/07391102.2019.1662850
معرّف المصادر الموحدhttp://hdl.handle.net/10576/31300
الملخصNickel oxide nanoparticles (NiO NPs) have received great interests in medical and biotechnological applications. However, their adverse impacts against biological systems have not been well-explored. Herein, the influence of NiO NPs on structural changes, heme degradation and aggregation of hemoglobin (Hb) was evaluated by UV-visible (Vis) spectroscopy, circular dichroism (CD) spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), and molecular modeling investigations. Also, the morphological changes and expression of Bax/Bcl-2 mRNA in human lymphocyte cell exposed to NiO NPs were assayed by DAPI staining and quantitative real-time PCR (qPCR), respectively. The UV-Vis study depicted that NiO NPs resulted in the displacement of aromatic residues and heme groups and production of the pro-aggregatory species. Intrinsic and Thioflavin T (ThT) fluorescence studies revealed that NiO NPs resulted in heme degradation and amorphous aggregation of Hb, respectively, which the latter result was also confirmed by TEM study. Moreover, far UV-CD study depicted that NiO NPs lead to substantial secondary structural changes of Hb. Furthermore, near UV-CD displayed that NiO NPs cause quaternary conformational changes of Hb as well as heme displacement. Molecular modelling study also approved that NiO NPs resulted in structural alterations of Hb and heme deformation. Moreover, morphological and genotoxicity assays revealed that the DNA fragmentation and expression ratio of Bax/Bcl-2 mRNA increased in lymphocyte cells treated with NiO NPs for 24hr. In conclusion, this study indicates that NiO NPs may affect the biological media and their applications should be limited. Communicated by Ramaswamy H. Sarma.
اللغةen
الناشرTaylor and Francis Ltd.
الموضوعaggregation
genotoxicity
heme degradation
hemoglobin
Interaction
nanoparticles
nickel oxide
العنوانThe effects of nickel oxide nanoparticles on structural changes, heme degradation, aggregation of hemoglobin and expression of apoptotic genes in lymphocytes
النوعArticle
رقم العدد12
رقم المجلد38
dc.accessType Abstract Only


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