Mesoporous silica coated carbon nanofibers reduce embryotoxicity via ERK and JNK pathways
View/ Open
Publisher version (Check access options)
Check access options
Date
2021Author
Abdo, Ghada G.Gupta, Ishita
Kheraldine, Hadeel
Rizeq, Balsam
Zagho, Moustafa M.
Khalil, Ashraf
Elzatahry, Ahmed
Al Moustafa, Ala-Eddin
...show more authors ...show less authors
Metadata
Show full item recordAbstract
Carbon nanofibers (CNFs) have been implicated in biomedical applications, yet, they are still considered as a potential hazard. Conversely, mesoporous silica is a biocompatible compound that has been used in various biomedical applications. In this regard, we recently reported that CNFs induce significant toxicity on the early stage of embryogenesis in addition to the inhibition of its angiogenesis. Thus, we herein use mesoporous silica coating of CNFs (MCNFs) in order to explore their outcome on normal development and angiogenesis using avian embryos at 3 days and its chorioallantoic membrane (CAM) at 6 days of incubation. Our data show that mesoporous silica coating of CNFs significantly reduces embryotoxicity provoked by CNFs. However, MCNFs exhibit slight increase in angiogenesis inhibition in comparison with CNFs. Further investigation revealed that MCNFs slightly deregulate the expression patterns of key controller genes involved in cell proliferation, survival, angiogenesis, and apoptosis as compared to CNFs. We confirmed these data using avian primary normal embryonic fibroblast cells established in our lab. Regarding the molecular pathways, we found that MCNFs downregulate the expression of ERK1/ERK2, p-ERK1/ERK2 and JNK1/JNK2/JNK3, thus indicating a protective role of MCNFs via ERK and JNK pathways. Our data suggest that coating CNFs with a layer of mesoporous silica can overcome their toxicity making them suitable for use in biomedical applications. Nevertheless, further investigations are required to evaluate the effects of MCNFs and their mechanisms using different in vitro and in vivo models. 2021 Elsevier B.V.
Collections
- Materials Science & Technology [293 items ]
- Medicine Research [1382 items ]
- Pharmacy Research [1234 items ]