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AuthorJina, Yammine
AuthorGharsallaoui, Adem
AuthorFadel, Alexandre
AuthorMechmechani, Samah
AuthorKaram, Layal
AuthorIsmail, Ali
AuthorChihib, Nour-Eddine
Available date2022-08-24T06:56:19Z
Publication Date2023-01-31
Publication NameFood Control
Identifierhttp://dx.doi.org/10.1016/j.foodcont.2022.109317
CitationYammine, J., Gharsallaoui, A., Fadel, A., Mechmechani, S., Karam, L., Ismail, A., & Chihib, N. E. (2022). Enhanced antimicrobial, antibiofilm and ecotoxic activities of nanoencapsulated carvacrol and thymol as compared to their free counterparts. Food Control, 109317.
ISSN09567135
URIhttps://www.sciencedirect.com/science/article/pii/S0956713522005102
URIhttp://hdl.handle.net/10576/33399
AbstractEssential oils active components emerged as captivating antimicrobials to control biofilms developed on food contact surfaces. Free and nanoencapsulated carvacrol (CAR) and thymol (THY) were assessed as antimicrobials against Salmonella Enteritidis biofilms adhered to stainless steel. The developed spherical nanocapsules using the spray-drying technique showed an average size ranged between 159.25 and 234.76 nm and zeta potential values ranged between 23.60 and 24.66 mV. The minimal inhibitory concentrations (MIC) of free THY and CAR were both 1.25 mg L−1. Nanoencapsulation reduced MIC values to 0.62 mg L−1 (THY) and 0.31 mg L−1 (CAR). Furthermore, the exposure to free and nanoencapsulated CAR and THY induced a destabilization of bacterial membranes with obvisous morphological deformations and a pronounced leakage of potassium ions and green fluorescent proteins. Eradication of S. Enteritidis biofilms developed on stainless steel was achieved following a 15 min treatment with nanoencapsulated CAR and THY at 2 MIC. Free antimicrobial exposures induced up to 4.27 log CFU cm−2 reductions. Additionally, the ecotoxicity tests against Daphnia magna crustaceans reported a non-toxicity of both free and nanoencapsulated CAR and THY after 48 h exposure. Thereby, both CAR and THY antimicrobials proved to be promising natural surface disinfectants that require further exploration and incorporation in food industries.
SponsorThis work was supported by the Partenariat Hubert Curien (PHC)- Cèdre program [42281SD]. The Chevreul Institute is also thanked for its help in the development of this work through the ARCHI-CM project supported by the “Ministère de l’Enseignement Supérieur de la Recherche et de l’Innovation”, the region “Hauts-de-France”, the ERDF program of the European Union and the “Métropole Européenne de Lille.
Languageen
PublisherElsevier
SubjectSalmonella enteritidis
Antibiofilm activity
Carvacrol
Thymol
Nanoencapsulation
Ecotoxicity
TitleEnhanced antimicrobial, antibiofilm and ecotoxic activities of nanoencapsulated carvacrol and thymol as compared to their free counterparts
TypeArticle
Volume Number143
dc.accessType Open Access


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