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المؤلفGhina, Hijazi
المؤلفDakroub, Fatima
المؤلفKhoueiry, Pierre
المؤلفEl-Kurdi, Abdullah
المؤلفEzzeddine, Amani
المؤلفAlkalamouni, Habib
المؤلفAlansari, Khalid
المؤلفAlthani, Asmaa A.
المؤلفMathew, Shilu
المؤلفAlKhatib, Hebah A.
المؤلفYassine, Hadi M.
المؤلفZaraket, Hassan
تاريخ الإتاحة2022-10-30T19:45:53Z
تاريخ النشر2022-11-30
اسم المنشورInfection, Genetics and Evolution
المعرّفhttp://dx.doi.org/10.1016/j.meegid.2022.105367
الاقتباسHijazi G, Dakroub F, Khoueiry P, El-Kurdi A, Ezzeddine A, Alkalamouni H, Alansari K, Althani AA, Mathew S, AlKhatib HA, Yassine HM, Zaraket H. Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar. Infect Genet Evol. 2022 Sep 14;105:105367. doi: 10.1016/j.meegid.2022.105367.
الرقم المعياري الدولي للكتاب15671348
معرّف المصادر الموحدhttps://www.sciencedirect.com/science/article/pii/S1567134822001642
معرّف المصادر الموحدhttp://hdl.handle.net/10576/35608
الملخصAcute gastroenteritis (AGE) is associated with significant global morbidity and mortality, especially among children under five years of age. Viruses are well established as etiologic agents of gastroenteritis since they are the most common pathogens that contribute to the disease burden in developing countries. Despite the advances in molecular diagnosis, a substantial proportion of AGE etiology remain unresolved. We implemented a viral metagenomics pipeline to determine the potential viral etiology associated with AGE among children under the age of five years in Qatar with undiagnosed etiology. Following enriching for the viral genome, ∼1.3 billion sequences were generated from 89 stool specimens using the Illumina HiSeq platform, of which 7% were mapped to viral genomes. Human viruses were detected in 34 specimens (38.2%); 14 were adenovirus, nine coxsackievirus A16, five rotavirus (G9P[8] and G4P[8]), four norovirus (GII), one influenza A virus (H3), and one respiratory syncytial virus A (RSVA). In conclusion, the viral metagenomics approach is useful for determining AGE's etiology when routine molecular diagnostic assays fail.
راعي المشروعThis study was supported by Qatar National Research Fund (QNRF) funding, grant # NPRP 9–133–1-025, and partial funding from Hamad Medical Corporation, grant # 16173/16.
اللغةen
الناشرElsevier
الموضوعGastroenteritis
Viral metagenomic
Next-generation sequencing
Unknown etiology
Viruses
العنوانViral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar
النوعArticle
رقم المجلد105
Open Access user License http://creativecommons.org/licenses/by/4.0/
dc.accessType Open Access


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