Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar
Author | Ghina, Hijazi |
Author | Dakroub, Fatima |
Author | Khoueiry, Pierre |
Author | El-Kurdi, Abdullah |
Author | Ezzeddine, Amani |
Author | Alkalamouni, Habib |
Author | Alansari, Khalid |
Author | Althani, Asmaa A. |
Author | Mathew, Shilu |
Author | AlKhatib, Hebah A. |
Author | Yassine, Hadi M. |
Author | Zaraket, Hassan |
Available date | 2022-10-30T19:45:53Z |
Publication Date | 2022-11-30 |
Publication Name | Infection, Genetics and Evolution |
Identifier | http://dx.doi.org/10.1016/j.meegid.2022.105367 |
Citation | Hijazi G, Dakroub F, Khoueiry P, El-Kurdi A, Ezzeddine A, Alkalamouni H, Alansari K, Althani AA, Mathew S, AlKhatib HA, Yassine HM, Zaraket H. Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar. Infect Genet Evol. 2022 Sep 14;105:105367. doi: 10.1016/j.meegid.2022.105367. |
ISSN | 15671348 |
Abstract | Acute gastroenteritis (AGE) is associated with significant global morbidity and mortality, especially among children under five years of age. Viruses are well established as etiologic agents of gastroenteritis since they are the most common pathogens that contribute to the disease burden in developing countries. Despite the advances in molecular diagnosis, a substantial proportion of AGE etiology remain unresolved. We implemented a viral metagenomics pipeline to determine the potential viral etiology associated with AGE among children under the age of five years in Qatar with undiagnosed etiology. Following enriching for the viral genome, ∼1.3 billion sequences were generated from 89 stool specimens using the Illumina HiSeq platform, of which 7% were mapped to viral genomes. Human viruses were detected in 34 specimens (38.2%); 14 were adenovirus, nine coxsackievirus A16, five rotavirus (G9P[8] and G4P[8]), four norovirus (GII), one influenza A virus (H3), and one respiratory syncytial virus A (RSVA). In conclusion, the viral metagenomics approach is useful for determining AGE's etiology when routine molecular diagnostic assays fail. |
Sponsor | This study was supported by Qatar National Research Fund (QNRF) funding, grant # NPRP 9–133–1-025, and partial funding from Hamad Medical Corporation, grant # 16173/16. |
Language | en |
Publisher | Elsevier |
Subject | Gastroenteritis Viral metagenomic Next-generation sequencing Unknown etiology Viruses |
Type | Article |
Volume Number | 105 |
Open Access user License | http://creativecommons.org/licenses/by/4.0/ |
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