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AuthorYu, Jianmei
AuthorGoktepe, Ipek
AuthorAhmedna, Mohamed
Available date2015-11-05T07:03:41Z
Publication Date2013
Publication NameInternational Journal of Food Science & Technology
ResourceWiley Online Library
CitationYu, J., Goktepe, I. and Ahmedna, M. (2013), Enzymatic treatment of peanut butter to reduce the concentration of major peanut allergens. International Journal of Food Science & Technology, 48: 1224-1234
ISSN1365-2621
URIhttp://dx.doi.org/10.1111/ijfs.12081
URIhttp://hdl.handle.net/10576/3651
AbstractThis study investigated the effects of enzymatic treatment of peanut butter on two-major peanut allergens (Ara h 1 and Ara h 2). Home-made and commercial peanut butter samples were treated with alpha-chymotrypsin, trypsin or the combination of these enzymes and incubated at room temperature for 24 h or at 37 °C for 3 h. Treated peanut butter samples were sampled weekly for evaluation of total soluble proteins and extractable Ara h 1/Ara h 2. Data show that 1:1 alpha-chymotrypsin: trypsin at 0.04% of enzyme-to-peanut butter ratio resulted in near complete reduction of extractable Ara h 1 and Ara h 2 respectively. Treatment of peanut butter with a combination of trypsin and alpha-chymotrypsin resulted in a decrease in IgE-binding, suggesting that enzymatic treatment has the potential to reduce the allergenicity. However, clinical tests are needed to confirm any reduction in allergenic potential. The amount of water used to disperse enzyme did not have significant effect on allergen reduction but affected the consistency and colour of treated products, especially when the amount of water added was above 5% of peanut butter weight.
Languageen
PublisherJohn Wiley & Sons, Ltd.
SubjectEnzymatic treatment
peanut butter
peanut allergen reduction
α‐chymotrypsin and trypsin
TitleEnzymatic treatment of peanut butter to reduce the concentration of major peanut allergens
TypeArticle
Issue Number6
Volume Number48
dc.accessType Abstract Only


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