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المؤلفBatista, F. M.
المؤلفLopez-Sanmartin, M.
المؤلفBoudry, P.
المؤلفNavas, J. I.
المؤلفRuano, F.
المؤلفRenault, T.
المؤلفFonseca, V. G.
المؤلفLeitao, A.
تاريخ الإتاحة2015-11-08T10:27:57Z
تاريخ النشر2014
اسم المنشورAquaculture Research
المصدرWiley Online Library
الاقتباسBatista, F. M., Lopez-Sanmartin, M., Boudry, P., Navas, J. I., Ruano, F., Renault, T., Fonseca, V. G. and Leitao, A. (2014), Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids. Aquaculture Research.
الرقم المعياري الدولي للكتاب1365-2109
معرّف المصادر الموحدhttp://dx.doi.org/10.1111/are.12613
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3785
الملخصCytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the OsHV-1 reference type. Moreover, in situ hybridization confirmed the presence of OsHV-1 in gill tissue. Oysters where OsHV-1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to OsHV-1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.
راعي المشروعFCT. Grant Number: SFRH/BD/8972/2002
راعي المشروعMarie Curie Training fellowship. Grant Numbers: PLUDAMOR, QLK5-CT-2000-60036
اللغةen
الناشرJohn Wiley & Sons Ltd
الموضوعaneuploidy
ostreid herpesvirus 1
oyster
Crassostrea
العنوانInsights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
النوعArticle
dc.accessType Abstract Only


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