Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis
المؤلف | Abdelkefi-Mesrati, Lobna |
المؤلف | Boukedi, Hanen |
المؤلف | Dammak-Karray, Mariam |
المؤلف | Sellami-Boudawara, Tahya |
المؤلف | Jaoua, Samir |
المؤلف | Tounsi, Slim |
تاريخ الإتاحة | 2015-12-24T05:26:35Z |
تاريخ النشر | 2011-02 |
اسم المنشور | Journal of Invertebrate Pathology |
المعرّف | http://dx.doi.org/10.1016/j.jip.2010.10.002 |
الاقتباس | "Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis", Abdelkefi-Mesrati, Lobna; Boukedi, Hanen; Dammak-Karray, Mariam; Sellami-Boudawara, Tahya; Jaoua, Samir et al, (2011) Journal of Invertebrate Pathology, vol. 106 (2) p. 250-254 |
الرقم المعياري الدولي للكتاب | 0022-2011 |
الملخص | The bacterium Bacillus thuringiensis produces, at the vegetative stage of its growth, Vip3A proteins with activity against a broad spectrum of lepidopteran insects. The Egyptian cotton leaf worm (Spodoptera littoralis) is an important agricultural pest that is susceptible to the Vip3Aa16 protein of Bacillus thuringiensis kurstaki strain BUPM95. The midgut histopathology of Vip3Aa fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formation in the apical region and cellular disintegration. Biotinylated Vip3Aa toxin bound proteins of 55- and 100-kDa on blots of S. littoralis brush border membrane preparations. These binding proteins differ in molecular size from those recognized by Cry1C, one of the very few Cry proteins active against the polyphagous S. littoralis. This result supports the use of Vip3Aa16 proteins as insecticidal agent, especially in case of Cry-resistance management. |
راعي المشروع | Ministère de l’Enseignement Supérieur et de la Recherche Scientifique |
اللغة | en |
الناشر | Elsevier Inc. |
الموضوع | Bacillus thuringiensis Spodoptera littoralis Vip3Aa16 Activation Histopathological effect Midgut putative receptors |
النوع | Article |
الصفحات | 250-254 |
رقم العدد | 2 |
رقم المجلد | 106 |
ESSN | 1096-0805 |
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