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AuthorSellami, Sameh
AuthorJamoussi, Kais
AuthorDabbeche, Emna
AuthorJaoua, Samir
Available date2015-12-24T09:28:08Z
Publication Date2011-09
Publication NameCurrent Microbiology
CitationSellami, Sameh, et al, (2011), "Increase of the Bacillus thuringiensis Secreted Toxicity Against Lepidopteron Larvae by Homologous Expression of the vip3LB", Current Microbiology,63(3): 289–294.
ISSN0343-8651
URIhttp://dx.doi.org/10.1007/s00284-011-9976-3
URIhttp://hdl.handle.net/10576/3987
AbstractThe Vegetative insecticidal Vip3A proteins display a wide range of insecticidal spectrum against several agricultural insect pests. The fact that the expression of vip3 genes occurs only during the vegetative growth phase of Bacillus thuringiensis is a limiting factor in term of production level. Therefore, extending the synthesis of the Vip proteins to the sporulation phase is a good alternative to reach high levels of toxin synthesis. In this study, we have demonstrated that the maximal production of the secreted Vip3LB (also called Vip3Aa16) during the growth of the wild-type strain B. thuringiensis BUPM 95 is reached at the end of the vegetative growth phase, and that the protein remains relatively stable in the culture supernatant during the late sporulation stages. The vip3LB gene was cloned and expressed under the control of the sporulation dependant promoters BtI and BtII in B. thuringiensis BUPM 106 (Vip3(-)) and BUPM 95 (Vip3(+)) strains. The examination of the culture supernatants during the sporulation phase evidenced the synthesis of Vip3LB and its toxicity against the second-instars larvae of the Lepidopteron insect Spodoptera littoralis for the recombinant BUPM 106. Moreover, there was an increase of the Vip3LB synthesis level and an enhancement of the oral toxicity for the recombinant BUPM 95 resulting from the expression of the vip3LB gene during both the vegetative and sporulation phases and the relative stability of the Vip3LB protein.
SponsorTunisian Ministere de l’Enseignement Superieur et de la Recherche Scientifique (MESRS)
Languageen
PublisherSpringer-Verlag
SubjectCulture Supernatant Protein
Sporulation Phase
Concentrate Culture Supernatant
Vegetative insecticidal proteins
Larval Body Weight
TitleIncrease of the Bacillus thuringiensis secreted toxicity against lepidopteron larvae by homologous expression of the vip3LB gene during sporulation stage
TypeArticle
Pagination289-294
Issue Number3
Volume Number63
ESSN1432-0991
dc.accessType Abstract Only


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