Detection and Phylogenetic Analysis of Human Pegivirus (GBV-C) Among Blood Donors and Patients Infected With Hepatitis B Virus (HBV) in Qatar
Date
2015-12Author
AbuOdeh, Raed O.Al-Absi, Enas
Ali, Nadima H.
Khalili, Makiyeh
Al-Mawlawi, Naema
Hadwan, Tameem A.
Al Thani, Asmaa A.
Nasrallah, Gheyath K.
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Metadata
Show full item recordAbstract
Human Pegivirus (HPgV), formerly GB virus-C/
Hepatitis G virus (GBV-C/HGV), collectively
known as GBV-C, is widely spread and has
been reported to be associated with non-A–E
hepatitis. To our knowledge, no previous study
was conducted about HPgV in Qatar. Thus, the
objectives of this study were as follows: (i) to
determine the rates of HPgV infection in Qatar
among healthy blood donors and HBV-infected
patients, and (ii) to determine the most predominant
HPgV genotype in Qatar. A total of
714 blood plasma samples from healthy donors
(612) and HBV-infected patients (102)
were collected. RNA was extracted, reversed
transcribed, and then subjected for HPgV detection
by two round-nested PCR using primers
amplifying a 208 bp of 50-UTR of the HPgV. For
genotyping, the 50-UTR PCR products (from 25
randomly picked samples) were cloned and
sequenced. The overall infection rate of HPgV
in Qatar was 13.3%. There was no significant
difference (P¼0.41) in the infection rates between
healthy donor (13.7%) and in HBVinfected
patients (10.7%). Moreover, we did not
find any significant association between HPgV
infection rates and nationality, sex, or age
(P>0.05). Sequence analysis of 40 50-UTR PCR
amplicons yielded the European genotype 2 as
most predominant in Qatar, although other
genotypes (5 and7) were also present. Our
results indicate that there is no strong correlation
between HPgV infection rate, condition,
nationality, age, and sex, and genotype 2 is
most predominant in Qatar
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