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المؤلفAli, Mohamed H.M.
المؤلفAl-Saad, Khalid A.
المؤلفAli, Carmen M.
تاريخ الإتاحة2016-03-24T13:39:02Z
تاريخ النشر2014-03
اسم المنشورPhysica Medica
المصدرScopus
المعرّفhttp://dx.doi.org/10.1016/j.ejmp.2013.06.002
الاقتباسAli, M.H.M., Al-Saad, K.A., Ali, C.M. "Biophysical studies of the effect of high power ultrasound on the DNA solution" (2014) Physica Medica, 30 (2), pp. 221-227.
الرقم المعياري الدولي للكتاب1120-1797
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4257
الملخصStability and molecular size of the DNA double helical structure were studied on an aqueous solution of DNA after exposure to high power doses of continuous wave ultrasound at frequency of 20kHz. Thermal transition spectrophotometry (UV-melting), constant-field gel electrophoresis (CFGE), differential scanning calorimetry (DSC) and dielectric properties measurements were used to evaluate the ultrasound-induced changes in the DNA double helical structure. The thermal transition spectrophotometry (UV-melting) and differential scanning calorimetry (DSC) results showed that ultrasound power caused loss of DNA double helical structure and the DNA double strands melting temperature decreased as the ultrasound power increased, indicating a decrease in the stability of the double helical structure of DNA. The constant-field gel electrophoresis (CFGE) results showed that the molecular size of the DNA fragments decreased as the ultrasound power increased. The dielectric data in the frequency range from 20Hz to 100kHz for the native DNA showed that dispersion at frequency of about 500Hz resulted from polarization induced by counterions. The decrease in the dielectric increment indicated a decrease in length of DNA molecule after exposure to ultrasound power.
اللغةen
الناشرElsevier B.V.
الموضوعDifferential scanning calorimetry (DSC) and dielectric properties measurements
DNA solution
Thermal transition spectrophotometry
Ultrasound
العنوانBiophysical studies of the effect of high power ultrasound on the DNA solution
النوعArticle
الصفحات221-227
رقم العدد2
رقم المجلد30
dc.accessType Abstract Only


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