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AuthorAbdel Samad, Rim
AuthorAl Disi, Zulfa
AuthorMohammad Ashfaq, Mohammad Yousaf
AuthorWahib, Sara Mohiddin
AuthorZouari, Nabil
Available date2023-06-01T07:31:32Z
Publication Date2020
Publication NameRSC Advances
ResourceScopus
URIhttp://dx.doi.org/10.1039/d0ra01229g
URIhttp://hdl.handle.net/10576/43692
AbstractOccurrence of mineral forming and other bacteria in mats is well demonstrated. However, their high diversity shown by ribotyping has not been explained, although it could explain the diversity of formed minerals. Common biomarkers as well as phylogenic relationships are useful tools for clustering the isolates and the prediction of their potential role in the natural niche. In this study, a combination of MALDI-TOF MS with PCA was shown to be a powerful tool to categorize 35 mineral forming bacterial isolates isolated from Dohat Faishakh sabkha, northwest of Qatar (23 from decaying mats and 12 from living ones). The 23 strains from decaying mats belong to the Virgibacillus genus as identified by ribotyping and are shown to be highly involved in the formation of protodolomite and a diversity of minerals. They were used as internal references for the categorization of sabkha bacteria. Combination of the isolation of bacteria on selective mineral forming media, their MALDI TOF MS protein profiling and PCA analysis established their relationship in a phylloproteomic dendrogram based on protein biomarkers including m/z 4905, 3265, 5240, 6430, 7765, and 9815. PCA analysis clustered the studied isolates into 3 major clusters, showing strong correspondence to the 3 phylloproteomic groups that were established by the dendrogram. Both clustering analysis means have evidently demonstrated a relationship between known Virgibacillus strains and other related bacteria based on profiling of their synthesized proteins. Thus, larger populations of bacteria in mats can be easily screened for their potential to exhibit certain activities, which is of ecological, environmental and biotechnological significance.
SponsorThis work was made possible by the QUST-1-CAS-2018-3 fund, a student grant from the Qatar University. Many thanks to Microbiology laboratory, Hamad Medical Corporation for providing facility for MALDI-TOF MS technique. The statements made herein are solely the responsibility of the authors.
Languageen
PublisherRoyal Society of Chemistry
SubjectBacteria
Bacteriology
Biomarkers
Biosynthesis
Data handling
Inductively coupled plasma
Minerals
Proteins
Bacillus species
Bacterial isolates
Clustering analysis
Isolation of bacteria
MALDI TOF MS
PCA analysis
Principle component analysis
Protein profiling
Principal component analysis
TitleThe use of principle component analysis and MALDI-TOF MS for the differentiation of mineral forming: Virgibacillus and Bacillus species isolated from sabkhas
TypeArticle
Pagination14606-14616
Issue Number25
Volume Number10
dc.accessType Open Access


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