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AuthorNasarallah, Gheyath K.
AuthorFakhroo, Aisha D.
AuthorKhan, Taushif
AuthorCyprian, Farhan S.
AuthorAl Ali, Fatima
AuthorAta, Manar M.A.
AuthorTaleb, Sara
AuthorZedan, Hadeel T.
AuthorAl-Sadeq, Duaa W.
AuthorAmanullah, Fathima H.
AuthorHssain, Ali A.
AuthorEid, Ali H.
AuthorAbu-Raddad, Laith J.
AuthorAl-Khal, Abdullatif
AuthorAl Thani, Asmaa A.
AuthorMarr, Nico
AuthorYassine, Hadi M.
Available date2023-07-11T07:33:59Z
Publication Date2022-10-29
Publication NameMediterranean Journal of Hematology and Infectious Diseases
Identifierhttp://dx.doi.org/10.4084/MJHID.2022.076
CitationNasarallah, G. K., Fakhroo, A. D., Khan, T., Cyprian, F. S., Al Ali, F., Ata, M. M., ... & Yassine, H. M. (2022). Detection of antinuclear antibodies targeting intracellular signal transduction, metabolism, apoptotic processes and cell death in critical COVID-19 patients. Mediterranean Journal of Hematology and Infectious Diseases, 14(1).
ISSN2035-3006
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85142793189&origin=inward
URIhttp://hdl.handle.net/10576/45348
AbstractBackground and Objectives: The heterogeneity of the coronavirus disease of 2019 (COVID-19) lies within its diverse symptoms and severity, ranging from mild to lethal. Acute respiratory distress syndrome (ARDS) is a leading cause of mortality in COVID-19 patients, characterized by a hyper cytokine storm. Autoimmunity is proposed to occur as a result of COVID-19, given the high similarity of the immune responses observed in COVID-19 and autoimmune diseases. Here, we investigate the level of autoimmune antibodies in COVID-19 patients with different severities. Results: Initial screening for antinuclear antibodies (ANA) IgG using ELISA revealed that 1.58% (2/126) and 4% (5/126) of intensive care unit (ICU) COVID-19 cases expressed strong and moderate ANA levels, respectively. An additional sample was positive with immunofluorescence assays (IFA) screening. However, all the non-ICU cases (n=273) were ANA negative using both assays. Samples positive for ANA were further confirmed with large-scale autoantibody screening by phage immunoprecipitation-sequencing (PhIP-Seq). The majority of the ANA-positive samples showed "speckled" ANA pattern by microscopy and revealed autoantibody specificities that targeted proteins involved in intracellular signal transduction, metabolism, apoptotic processes, and cell death by PhIP-Seq; further denoting reactivity to nuclear and cytoplasmic antigens. Conclusion: Our results further support the notion of routine screening for autoimmune responses in COVID-19 patients, which might help improve disease prognosis and patient management. Further, results provide compelling evidence that ANA-positive individuals should be excluded from being donors for convalescent plasma therapy in the context of COVID-19.
SponsorThis study was supported by funds from QNRF, grant # NPRP11S-1212-170092.
Languageen
PublisherUniversita Cattolica del Sacro Cuore
SubjectANA
Autoimmunity
Coronavirus
COVID-19
ICU
TitleDetection of Antinuclear Antibodies Targeting Intracellular Signal Transduction, Metabolism, Apoptotic Processes and Cell Death in Critical COVID-19 Patients
TypeArticle
Issue Number1
Volume Number14
ESSN2035-3006
dc.accessType Open Access


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