عرض بسيط للتسجيلة

المؤلفAlsalabi, Fatma Ali
المؤلفHassan, Zahoor Ul
المؤلفAl-Thani, Roda F.
المؤلفJaoua, Samir
تاريخ الإتاحة2023-07-26T09:42:43Z
تاريخ النشر2023-01-01
اسم المنشورHeliyon
المعرّفhttp://dx.doi.org/10.1016/j.heliyon.2023.e12835
الاقتباسAlsalabi, F. A., Hassan, Z. U., Al-Thani, R. F., & Jaoua, S. (2023). Molecular identification and biocontrol of ochratoxigenic fungi and ochratoxin A in animal feed marketed in the state of Qatar. Heliyon, 9(1).‏
الرقم المعياري الدولي للكتاب24058440
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85146070070&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/46349
الملخصOchratoxin A (OTA) is a toxic fungal metabolite produced by some Aspergillus and Penicillium species. This work was designed to explore the presence of OTA and ochratoxigenic fungi in feed grains marketed in Qatar and their biological control by a bacterium (Burkholderia cepacia). Significantly higher levels of OTA were detected in mixed grains samples (144.59 ± 6.63 μg/kg), compared to the maize (25.27 ± 1.89 μg/kg) and wheat (3.37 ± 0.11 μg/kg). OTA-producing fungi (A. niger, A. ochraceus, A. westerdijkiae, A. carbonarius and P. verrucosum) were identified on the basis of their morphological features as well as through polymerase chain reaction (PCR). Putative ochratoxigenic polyketide genes in these isolates were evidenced by using primers AoOTA-L/AoOTA-R (in A. ochraceus and A. westerdijkiae), AoPks1/AoPks2 (in A. niger and A. ochraceus) and PenPks1/Penpks2 (in P. verrucosum). On synthetic media, A. westerdijkiae showed the highest OTA synthesis (5913 ± 576 μg/kg) than the closely related A. ochraceus (3520 ± 303 μg/kg), A. carbonarius (3064 ± 289 μg/kg) and P. verrucosum (3030 ± 710 μg/kg). Burkholderia cepacia cells and culture extract showed promising biological control potentials against OTA producing fungi. On the basis of these findings, it can be concluded that animal feed samples are generally contaminated with OTA-producing fungi as well as OTA, and Burkholderia cepacia CS5 exhibits promising antifungal activities.
راعي المشروعWe are very grateful to the Centre of Biotechnology of Sfax (Tunisia) “Laboratory of Biopesticides” for providing us with Burkholderia cepacia strain CS5. Open Access funding is provided by the Qatar National Library.
اللغةen
الناشرElsevier Ltd
الموضوعAnimal feed
Biocontrol
Molecular identification
Ochratoxin A
Qatar
Toxigenic fungi
العنوانMolecular identification and biocontrol of ochratoxigenic fungi and ochratoxin A in animal feed marketed in the state of Qatar
النوعArticle
الصفحات2-10
رقم العدد1
رقم المجلد9
dc.accessType Open Access


الملفات في هذه التسجيلة

Thumbnail

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة