Deletion of potD, encoding a putative spermidine-binding protein, results in a complex phenotype in Legionella pneumophila
المؤلف | Nasrallah, Gheyath K. |
المؤلف | Abdelhady, Hany |
المؤلف | Tompkins, Nicholas P. |
المؤلف | Carson, Kaitlyn R. |
المؤلف | Garduno, Rafael A. |
تاريخ الإتاحة | 2016-09-21T08:12:10Z |
تاريخ النشر | 2014-07 |
اسم المنشور | International Journal of Medical Microbiology |
المصدر | Scopus |
المعرّف | http://dx.doi.org/10.1016/j.ijmm.2014.05.004 |
الاقتباس | Nasrallah, G.K., Abdelhady, H., Tompkins, N.P., Carson, K.R., Garduno, R.A. "Deletion of potD, encoding a putative spermidine-binding protein, results in a complex phenotype in Legionella pneumophila" (2014) International Journal of Medical Microbiology, 304 (5-6), pp. 703-716. |
الرقم المعياري الدولي للكتاب | 1438-4221 |
الملخص | L. pneumophila is an intracellular pathogen that replicates in a membrane-bound compartment known as the Legionella-containing vacuole (LCV). We previously observed that the polyamine spermidine, produced by host cells or added exogenously, enhances the intracellular growth of L. pneumophila. To study this enhancing effect and determine whether polyamines are used as nutrients, we deleted potD from L. pneumophila strain JR32. The gene potD encodes a spermidine-binding protein that in other bacteria is essential for the function of the PotABCD polyamine transporter. Deletion of potD did not affect L. pneumophila growth in vitro in the presence or absence of spermidine and putrescine, suggesting that PotD plays a redundant or no role in polyamine uptake. However, deletion of potD resulted in a puzzlingly complex phenotype that included defects in L. pneumophila's ability to form filaments, tolerate Na+, associate with macrophages and amoeba, recruit host vesicles to the LCV, and initiate intracellular growth. Moreover, the ?potD mutant was completely unable to grow in L929 cells treated with a pharmacological inhibitor of spermidine synthesis. These complex and disparate effects suggest that the L. pneumophila potD encodes either: (i) a multifunctional protein, (ii) a protein that interacts with, or regulates a, multifunctional protein, or (iii) a protein that contributes (directly or indirectly) to a regulatory network. Protein function studies with the L. pneumophila PotD protein are thus warranted. |
راعي المشروع | Discovery Grant to RAG from the Natural Sciences and Engineering Research Council (NSERC) of Canada and a start-up Grant to GKN from Qatar University. |
اللغة | en |
الناشر | Elsevier GmbH |
الموضوع | Legionella Pathogenesis Polyamines PotD Spermidine-binding protein |
النوع | Article |
الصفحات | 703-716 |
رقم العدد | 5--6 |
رقم المجلد | 304 |
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