Medicago orbicularis Has Antioxidant, Antihemolytic, and Anti-cancerous Activities and Augments Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells
المؤلف | Shaito, Abdullah A. |
المؤلف | Omairi, Islam |
المؤلف | Al-Thani, Najlaa |
المؤلف | Seglab, Fatiha |
المؤلف | Ad-darwish, Esraa |
المؤلف | Kobeissy, Firas H. |
المؤلف | Nasreddine, Salam |
تاريخ الإتاحة | 2023-09-21T08:32:23Z |
تاريخ النشر | 2023 |
اسم المنشور | Medicine and Pharmacology, Complementary and Alternative Medicine |
المعرّف | http://dx.doi.org/10.20944/preprints202309.1208.v1 |
الاقتباس | Shaito, A.A.; Omairi, I.; Al-Thani, N.; Seglab, F.; Ad-darwish, E.; Kobeissy, F.H.; Nasreddine, S. Medicago orbicularis Has Antioxidant, Antihemolytic, and Anti-cancerous Activities and Augments Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells. Preprints 2023, 2023091208. https://doi.org/10.20944/preprints202309.1208.v1 |
الملخص | Cancer is the second leading cause of death, worldwide. Lung cancer is the leading cause of cancer-related mortality. Plant-based therapeutics and herbal medicine have played a vital role in the development of several anti-cancerous agents, and has been used to reduce the severe side effects of chemotherapy as well. Since the anti-lung cancer properties of the plant Medicago. orbicularis are not explored yet, we identified its phytochemical composition and investigated the anti-oxidant, anti-hemolytic, and anti-cancerous properties of extracts of this plant in A549 human lung adenocarcinoma cells. Results show that all parts of M. orbicularis (stems, leaves, and fruits) exhibit remarkable anti-oxidant and hemolytic activities. In addition, all extracts showed a dose-dependent anti-cancerous cytotoxic activity against A549 cells; with fruit extracts being the most potent. This cytotoxic effect could be related, at least partly, to the induction of apoptosis, where M. orbicularis fruit extracts activated Caspase-3 and PARPP-1, and reduced the ratio of anti-apoptotic BCL-2/ pro-apoptotic BAX, thereby promoting cellular death. Furthermore, the use of M. orbicularis, in combination with a conventional chemotherapeutic agent, cisplatin, was assessed. Indeed, combination of cisplatin and M. orbicularis fruit extracts was more cytotoxic and induced more aggregation of A549 cells than either treatment alone. GC-MS analysis and total polyphenol and flavonoid content determination indicated that M. orbicularis is rich in compounds that have anti-cancerous effects. M. orbicularis may be a potential source of anti-cancerous agents to manage progression of lung cancer and its resistance to therapy. |
راعي المشروع | This work was supported by the a grant from the Lebanese University to SN and student grants number QUST-1-BRC-2022-315; QUST-1-BRC-2022-316, QUST-1-BRC-2023-836; and QUST-1-BRC-2023-846 to AS. Publication fees APC were covered by Qatar National Library (QNL). |
اللغة | en |
الناشر | preprints |
الموضوع | Medicago orbicularis Lung cancer Herbal medicine Antioxidants hemolytic cisplatin A549 cells |
النوع | Article |
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