Platelets as a Possible Reservoir of HCV And Predictor of Response to Treatment
التاريخ
2016المؤلف
Amer, AliaaAbu Madi, Marawan
Shebl, Fatma
Al Faridi, Dekra
Alkhinji, Moza
Derbala, Moutaz
...show more authors ...show less authors
البيانات الوصفية
عرض كامل للتسجيلةالملخص
In the era of new Hepatitis C Virus (HCV) therapy, and the detection of
extrahepatic HCV reservoirs such as peripheral blood mononuclear
cells and platelets, it is important to understand the factors underlying
resistance to treatment. Detection and quantitation of HCV-RNA
in platelets or leucocytes from patients under antiviral therapy is
poorly studied and the limited studies generated contradictory
results.
Aim: To detect and quantify HCV-RNA in platelets, and to evaluate
the relation between HCV-RNA in the serum and the kinetics of
HCV-RNA in platelets, in response to treatment.
Method: Viral kinetic was tested in 20 chronic HCV genotype4,
during the course of therapy.
Results: HCV-RNA was detected in sera of all infected patients.
The baseline platelet viral load was significantly lower in responders
compared to non-responders. Platelet viral load was also related to
serum viral load (t=3.39, p=0.001), but not related to platelet count
(t=-0.56, p=0.58). ROC curve analysis revealed that in general,
platelet viral load at different time points was a better predictor of
SVR compared to serum viral load.
Conclusion: HCV RNA analysis in whole blood may be more
sensitive than platelet-poor plasma, which might underestimate
circulating viral load. Early eradication of viremia from platelets is
associated with higher rates of SVR. Our data, reconfirm higher
HCV-RNA levels in serum compared to platelets. Thrombocytopenia
occurring during interferon-based therapy might be a manifestation
of viral eradication rather than adverse effects. Our findings warrant
testing the sensitivity of platelet viral load as a predictor of poor
response.
DOI/handle
http://dx.doi.org/10.4172/2324-8955.1000157http://hdl.handle.net/10576/4809
http://dx.doi.org/10.4172/2324-8955.1000157
المجموعات
- الأبحاث [709 items ]
- العلوم الحيوية الطبية [692 items ]