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AuthorAl Yafei, Zain
AuthorHajjej, Abdelhafidh
AuthorAlvares, Marion
AuthorAl Mahri, Ayeda
AuthorNasr, Amre
AuthorMirghani, Rajaa
AuthorAl Obaidli, Ali
AuthorAl Seiari, Mohamed
AuthorMack, Steven J.
AuthorAskar, Medhat
AuthorEdinur, Hisham A.
AuthorAlmawi, Wassim Y.
AuthorElGhazali, Gehad
Available date2023-11-19T05:45:35Z
Publication Date2023
Publication NameGenes
ResourceScopus
ISSN20734425
URIhttp://dx.doi.org/10.3390/genes14061159
URIhttp://hdl.handle.net/10576/49440
AbstractIn this study, we investigated HLA class I and class II allele and haplotype frequencies in Emiratis and compared them to those of Asian, Mediterranean, and Sub-Saharan African populations. Methods: Two-hundred unrelated Emirati parents of patients selected for bone marrow transplantation were genotyped for HLA class I (A, B, C) and class II (DRB1, DQB1) genes using reverse sequence specific oligonucleotide bead-based multiplexing. HLA haplotypes were assigned with certainty by segregation (pedigree) analysis, and haplotype frequencies were obtained by direct counting. HLA class I and class II frequencies in Emiratis were compared to data from other populations using standard genetic distances (SGD), Neighbor-Joining (NJ) phylogenetic dendrograms, and correspondence analysis. Results: The studied HLA loci were in Hardy-Weinberg Equilibrium. We identified 17 HLA-A, 28 HLA-B, 14 HLA-C, 13 HLA-DRB1, and 5 HLA-DQB1 alleles, of which HLA-A*02 (22.2%), -B*51 (19.5%), -C*07 (20.0%), -DRB1*03 (22.2%), and -DQB1*02 (32.8%) were the most frequent allele lineages. DRB1*03~DQB1*02 (21.2%), DRB1*16~DQB1*05 (17.3%), B*35~C*04 (11.7%), B*08~DRB1*03 (9.7%), A*02~B*51 (7.5%), and A*26~C*07~B*08~DRB1*03~DQB1*02 (4.2%) were the most frequent two- and five-locus HLA haplotypes. Correspondence analysis and dendrograms showed that Emiratis were clustered with the Arabian Peninsula populations (Saudis, Omanis and Kuwaitis), West Mediterranean populations (North Africans, Iberians) and Pakistanis, but were distant from East Mediterranean (Turks, Albanians, Greek), Levantine (Syrians, Palestinians, Lebanese), Iranian, Iraqi Kurdish, and Sub-Saharan populations. Conclusions: Emiratis were closely related to Arabian Peninsula populations, West Mediterranean populations and Pakistanis. However, the contribution of East Mediterranean, Levantine Arab, Iranian, and Sub-Saharan populations to the Emiratis' gene pool appears to be minor.
SponsorThis research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors in the UAE. This work was supported by the National Institutes of Health (NIH) and National Institute of Allergy and Infectious Disease (NIAID), grant R01AI128775 (SJM). The content is solely the responsibility of the authors and does not necessarily reflect the official views of the NIAID, NIH, and United States government.
Languageen
PublisherMDPI
Subjectallele
Arabs
Emiratis
genotyping
haplotypes
HLA
human leukocyte antigen
TitleAnalysis of the Origin of Emiratis as Inferred from a Family Study Based on HLA-A, -C, -B, -DRB1, and -DQB1 Genes
TypeArticle
Issue Number6
Volume Number14
dc.accessType Open Access


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