Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease
Author | Firouzamandi, Masoumeh |
Author | Moeini, Hassan |
Author | Hosseini, Seyed Davood |
Author | Bejo, Mohd Hair |
Author | Omar, Abdul Rahman |
Author | Mehrbod, Parvaneh |
Author | El Zowalaty, Mohamed E. |
Author | Webster, Thomas J. |
Author | Ideris, Aini |
Available date | 2016-11-01T10:43:08Z |
Publication Date | 2016-01-14 |
Publication Name | International Journal of Nanomedicine |
Identifier | https://dx.doi.org/10.2147/IJN.S92225 |
Citation | Firouzamandi M, Moeini H, Hosseini SD, et al. Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease. International Journal of Nanomedicine. 2016;11:259-267. |
ISSN | 1176-9114 |
Abstract | Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 µg pDNA/egg alone induced high levels of antibody titer (P<0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 µg pDNA + 64 µg D-SPM and 40 µg pDNA at 4 weeks postvaccination (P>0.05). Higher antibody titer was observed in the group immunized with 40 µg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 µg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery. |
Sponsor | Ministry of Science, Technology and Innovations (MOSTI), Malaysia, for the research grant (ERGS/1-2012/5527122), and Institute of Bioscience, Higher Institution Centre of Excellence (IBS HICoE) grant from the Ministry of Higher Education, Government of Malaysia |
Language | en |
Publisher | Dove Press |
Subject | Newcastle disease DNA vaccine in ovo vaccination Newcastle disease virus dextran-spermine nanoparticle hemagglutinin and fusion |
Type | Article |
Pagination | 259-267 |
Volume Number | 11 |
ESSN | 1178-2013 |
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