Show simple item record

AuthorSonia, Boughattas
AuthorAl-Khater, Albandari
AuthorAlbatesh, Dana
AuthorGiraldes, Bruno W
AuthorAbu-Madi, Marawan
AuthorAlthani, Asma A.
AuthorBenslimane, Fatiha M.
Available date2024-01-15T07:51:24Z
Publication Date2024-01-31
Publication NameEcological Indicators
Identifierhttp://dx.doi.org/10.1016/j.ecolind.2023.111298
ISSN1470160X
URIhttps://www.sciencedirect.com/science/article/pii/S1470160X23014401
URIhttp://hdl.handle.net/10576/50717
AbstractSponges play important role within aquatic ecosystems due to their diverse abilities including filter-based feeding mechanisms. Hence, this study evaluated the potential use of sponges as ecological biomonitors for water safety surveillance, especially in the presence of Waterborne protozoan pathogens WBPP. Sponge specimens were collected from different Qatari marine ecosystems and subjected to gDNA extraction and real-time PCR using specific primer sets for the most common WBPP. Two sponges from the coastal marine ecosystems were found to be positive for Blastocystis sp., and one sponge was positive for Dientamoeba fragilis within offshore site. No Cryptosporidium spp., Giardia duodenalis, nor Toxoplasma gondii were detected. Further genotyping analysis revealed that the Blastocystis sp. positive samples were subtype ST3 (allele 34), which matched local clinical isolates and D. fragilis specimen was unambiguously clustering with Genotype 2. In conclusion, this study demonstrates the role of marine sponges as ecological biomonitors for WBPP screening and provide insights into these pathogens widespread and their potential transmission to marine and terrestrial organisms including human.
Languageen
PublisherElsevier
SubjectMarine Demospongiae
Waterborne protozoan pathogens
qPCR
Sequencing
TitleMolecular investigation of waterborne protozoan contamination using marine Demospongiae
TypeArticle
Volume Number158
Open Access user License http://creativecommons.org/licenses/by/4.0/
ESSN1872-7034
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record