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المؤلفZghal, Raida Zribi
المؤلفElleuch, Jihen
المؤلفAli, Mamdouh Ben
المؤلفDarriet, Frederic
المؤلفRebaï, Ahmed
المؤلفChandre, Fabrice
المؤلفJaoua, Samir
المؤلفTounsi, Slim
تاريخ الإتاحة2017-02-19T08:47:15Z
تاريخ النشر2017-01
اسم المنشورApplied Microbiology and Biotechnology
المعرّفhttp://dx.doi.org/10.1007/s00253-016-7766-3
الاقتباسZghal, R.Z., Elleuch, J., Ben Ali, M. et al. "Towards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin" Appl Microbiol Biotechnol (2017) 101: 113.
الرقم المعياري الدولي للكتاب0175-7598
معرّف المصادر الموحدhttp://hdl.handle.net/10576/5278
الملخصAttempts have been made to express or to merge different Cry proteins in order to enhance toxic effects against various insects. Cry1A proteins of Bacillus thuringiensis form a typical bipyramidal parasporal crystal and their protoxins contain a highly conserved C-terminal region. A chimerical gene, called cry(4Ba-1Ac), formed by a fusion of the N-terminus part of cry4Ba and the C-terminus part of cry1Ac, was constructed. Its transformation to an acrystalliferous B. thuringiensis strain showed that it was expressed as a chimerical protein of 116 kDa, assembled in spherical to amorphous parasporal crystals. The chimerical gene cry(4Ba-1Ac) was introduced in a B. thuringiensis kurstaki strain. In the generated crystals of the recombinant strain, the presence of Cry(4Ba-1Ac) was evidenced by MALDI-TOF. The recombinant strain showed an important increase of the toxicity against Culex pipiens larvae (LC50 = 0.84 mg l−1 ± 0.08) compared to the wild type strain through the synergistic activity of Cry2Aa with Cry(4Ba-1Ac). The enhancement of toxicity of B. thuringiensis kurstaki expressing Cry(4Ba-1Ac) compared to that expressing the native toxin Cry4Ba, might be related to its a typical crystallization properties. The developed fusion protein could serve as a potent toxin against different pests of mosquitoes and major crop plants.
راعي المشروعThis work was supported by grants from the Tunisian Ministry of Higher Education and Scientific Research.
اللغةen
الناشرSpringer-Verlag
الموضوعBacillus thuringiensis kurstaki
Chimerical protein Cry(4Ba-1Ac)
Toxicity improvement to Culex pipiens
العنوانTowards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin
النوعArticle
الصفحات113-122
رقم العدد1
رقم المجلد101
ESSN1432-0614
dc.accessType Abstract Only


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