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المؤلفPatil, Veerupaxagouda
المؤلفHernandez-Franco, Juan F.
المؤلفYadagiri, Ganesh
المؤلفBugybayeva, Dina
المؤلفDolatyabi, Sara
المؤلفFeliciano-Ruiz, Ninoshkaly
المؤلفSchrock, Jennifer
المؤلفSuresh, Raksha
المؤلفHanson, Juliette
المؤلفYassine, Hadi
المؤلفHogenEsch, Harm
المؤلفRenukaradhya, Gourapura J.
تاريخ الإتاحة2024-03-13T05:14:56Z
تاريخ النشر2023
اسم المنشورVaccines
المصدرScopus
الرقم المعياري الدولي للكتاب2076393X
معرّف المصادر الموحدhttp://dx.doi.org/10.3390/vaccines11111707
معرّف المصادر الموحدhttp://hdl.handle.net/10576/52971
الملخصSwine influenza A viruses (SwIAVs) are pathogens of both veterinary and medical significance. Intranasal (IN) vaccination has the potential to reduce flu infection. We investigated the efficacy of split SwIAV H1N2 antigens adsorbed with a plant origin nanoparticle adjuvant [Nano11–SwIAV] or in combination with a STING agonist ADU-S100 [NanoS100–SwIAV]. Conventional pigs were vaccinated via IN and challenged with a heterologous SwIAV H1N1-OH7 or 2009 H1N1 pandemic virus. Immunologically, in NanoS100–SwIAV vaccinates, we observed enhanced frequencies of activated monocytes in the blood of the pandemic virus challenged animals and in tracheobronchial lymph nodes (TBLN) of H1N1-OH7 challenged animals. In both groups of the virus challenged pigs, increased frequencies of IL-17A+ and CD49d+IL-17A+ cytotoxic lymphocytes were observed in Nano11–SwIAV vaccinates in the draining TBLN. Enhanced frequency of CD49d+IFNγ+ CTLs in the TBLN and blood of both the Nano11-based SwIAV vaccinates was observed. Animals vaccinated with both Nano11-based vaccines had upregulated cross-reactive secretory IgA in the lungs and serum IgG against heterologous and heterosubtypic viruses. However, in NanoS100–SwIAV vaccinates, a slight early reduction in the H1N1 pandemic virus and a late reduction in the SwIAV H1N1-OH7 load in the nasal passages were detected. Hence, despite vast genetic differences between the vaccine and both the challenge viruses, IN vaccination with NanoS100–SwIAV induced antigen-specific moderate levels of cross-protective immune responses.
راعي المشروعThis work was supported by the USDA-NIFA AFRI grant #2019-67015-29814. The anti-porcine CXCL10 and IL-17A MAbs were provided by Dr Joan Lunney through efforts of the USDA-NIFA AFRI grant # 2019-67015-29815. Salaries and research support were provided by state and federal funds appropriated to OARDC, The Ohio State University, and Hatch formula funds from USDA-NIFA (Project No. IND020164H).
اللغةen
الناشرMultidisciplinary Digital Publishing Institute (MDPI)
الموضوعADU-S100
cell-mediated immune responses
intranasal vaccination
memory responses
Nano11
swine
swine influenza A virus
العنوانCharacterization of the Efficacy of a Split Swine Influenza A Virus Nasal Vaccine Formulated with a Nanoparticle/STING Agonist Combination Adjuvant in Conventional Pigs
النوعArticle
رقم العدد11
رقم المجلد11
dc.accessType Open Access


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