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AuthorPatil, Veerupaxagouda
AuthorHernandez-Franco, Juan F.
AuthorYadagiri, Ganesh
AuthorBugybayeva, Dina
AuthorDolatyabi, Sara
AuthorFeliciano-Ruiz, Ninoshkaly
AuthorSchrock, Jennifer
AuthorSuresh, Raksha
AuthorHanson, Juliette
AuthorYassine, Hadi
AuthorHogenEsch, Harm
AuthorRenukaradhya, Gourapura J.
Available date2024-03-13T05:14:56Z
Publication Date2023
Publication NameVaccines
ResourceScopus
ISSN2076393X
URIhttp://dx.doi.org/10.3390/vaccines11111707
URIhttp://hdl.handle.net/10576/52971
AbstractSwine influenza A viruses (SwIAVs) are pathogens of both veterinary and medical significance. Intranasal (IN) vaccination has the potential to reduce flu infection. We investigated the efficacy of split SwIAV H1N2 antigens adsorbed with a plant origin nanoparticle adjuvant [Nano11–SwIAV] or in combination with a STING agonist ADU-S100 [NanoS100–SwIAV]. Conventional pigs were vaccinated via IN and challenged with a heterologous SwIAV H1N1-OH7 or 2009 H1N1 pandemic virus. Immunologically, in NanoS100–SwIAV vaccinates, we observed enhanced frequencies of activated monocytes in the blood of the pandemic virus challenged animals and in tracheobronchial lymph nodes (TBLN) of H1N1-OH7 challenged animals. In both groups of the virus challenged pigs, increased frequencies of IL-17A+ and CD49d+IL-17A+ cytotoxic lymphocytes were observed in Nano11–SwIAV vaccinates in the draining TBLN. Enhanced frequency of CD49d+IFNγ+ CTLs in the TBLN and blood of both the Nano11-based SwIAV vaccinates was observed. Animals vaccinated with both Nano11-based vaccines had upregulated cross-reactive secretory IgA in the lungs and serum IgG against heterologous and heterosubtypic viruses. However, in NanoS100–SwIAV vaccinates, a slight early reduction in the H1N1 pandemic virus and a late reduction in the SwIAV H1N1-OH7 load in the nasal passages were detected. Hence, despite vast genetic differences between the vaccine and both the challenge viruses, IN vaccination with NanoS100–SwIAV induced antigen-specific moderate levels of cross-protective immune responses.
SponsorThis work was supported by the USDA-NIFA AFRI grant #2019-67015-29814. The anti-porcine CXCL10 and IL-17A MAbs were provided by Dr Joan Lunney through efforts of the USDA-NIFA AFRI grant # 2019-67015-29815. Salaries and research support were provided by state and federal funds appropriated to OARDC, The Ohio State University, and Hatch formula funds from USDA-NIFA (Project No. IND020164H).
Languageen
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
SubjectADU-S100
cell-mediated immune responses
intranasal vaccination
memory responses
Nano11
swine
swine influenza A virus
TitleCharacterization of the Efficacy of a Split Swine Influenza A Virus Nasal Vaccine Formulated with a Nanoparticle/STING Agonist Combination Adjuvant in Conventional Pigs
TypeArticle
Issue Number11
Volume Number11
dc.accessType Open Access


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