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Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae

المؤلفHanen, Boukedi
المؤلفBen Khedher, Saoussen
المؤلفTriki, Nesrine
المؤلفKamoun, Fakher
المؤلفSaadaoui, Imen
المؤلفChakroun, Maissa
المؤلفTounsi, Slim
المؤلفAbdelkefi-Mesrati, Lobna
تاريخ الإتاحة2024-04-02T04:00:53Z
تاريخ النشر2015-05-01
اسم المنشورJournal of Invertebrate Pathology
المعرّفhttp://dx.doi.org/10.1016/j.jip.2015.03.013
الاقتباسBoukedi, H., Khedher, S. B., Triki, N., Kamoun, F., Saadaoui, I., Chakroun, M., ... & Abdelkefi-Mesrati, L. (2015). Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae. Journal of invertebrate pathology, 127, 127-129.
الرقم المعياري الدولي للكتاب0022-2011
معرّف المصادر الموحدhttps://www.sciencedirect.com/science/article/pii/S0022201115000683
معرّف المصادر الموحدhttp://hdl.handle.net/10576/53780
الملخصThe vip3Aa16 gene of Bacillus thuringiensis strain BUPM95 was cloned and expressed in Escherichia coli. Optimization of Vip3A16 protein expression was conducted using Plackett–Burman design and response surface methodology. Accordingly, the optimum Vip3A16 toxin production was 170μg/ml at 18h post-induction time and 39°C post-induction temperature. This corresponds to an improvement of 21times compared to the starting conditions. The insecticidal activity, evaluated against Ectomyelois ceratoniae, displayed an LC50 value of 40ng/cm2 and the midgut histopathology of Vip3Aa16 fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formation in the apical region and cellular disintegration.
راعي المشروعThis study was supported by grants from the Ministry of Higher Education and Scientific Research .
اللغةen
الناشرElsevier
الموضوعVip3Aa16
Bacillus thuringiensis
Escherichia coli
Toxicity
Ectomyelois ceratoniae
العنوانOverproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae
النوعArticle
الصفحات127-129
رقم المجلد127
ESSN1096-0805
dc.accessType Abstract Only


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