A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
Abstract
Abstract The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.
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