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AuthorMansour, Amira
AuthorTammam, Salma
AuthorAlthani, Asma
AuthorAzzazy, Hassan M.E.
Available date2017-07-30T07:32:46Z
Publication Date2017-08-01
Publication NameJournal of Microbiological Methods
Identifierhttp://dx.doi.org/10.1016/j.mimet.2017.06.001
CitationMansour A, Tammam S, Althani A, Azzazy HME. A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay. Journal of Micobiological Methods. Vol. 139, P.p. 139.
ISSN01677012
URIhttp://www.sciencedirect.com/science/article/pii/S0167701217301495
URIhttp://hdl.handle.net/10576/5605
AbstractAbstract The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.
SponsorThis report was made possible by a NPRP award [NPRP 4-1215-3- 317] from the Qatar National Research Fund (a member of The Qatar Foundation).
Languageen
PublisherElsevier
SubjectMycobacterium tuberculosis
Fluorescence Resonance Energy Transfer
Gold nanoparticles
16s rDNA
In vitro diagnosis
TitleA single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
TypeArticle
Pagination165-167
Volume Number139
ESSN1872-8359
dc.accessType Abstract Only


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