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المؤلفZinellu, Angelo
المؤلفSotgiu, Elisabetta
المؤلفAssaretti, Stefano
المؤلفSotgia, Salvatore
المؤلفPaliogiannis, Panagiotis
المؤلفPintus, Gianfranco
المؤلفMangoni, Arduino A.
المؤلفCarru, Ciriaco
تاريخ الإتاحة2018-02-04T11:48:08Z
تاريخ النشر2017-12-03
اسم المنشورJournal of Analytical Methods in Chemistry
المعرّفhttp://dx.doi.org/10.1155/2017/4065892
الاقتباسAngelo Zinellu, Elisabetta Sotgiu, Stefano Assaretti, et al., “Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection,” Journal of Analytical Methods in Chemistry, vol. 2017, Article ID 4065892, 6 pages, 2017. doi:10.1155/2017/4065892
الرقم المعياري الدولي للكتاب2090-8865
المعرّفArticle ID 4065892
معرّف المصادر الموحدhttp://hdl.handle.net/10576/6192
الملخصAlterations in global DNA methylation are implicated in various pathophysiological processes. The development of simple and quick, yet robust, methods to assess DNA methylation is required to facilitate its measurement and interpretation in clinical practice. We describe a highly sensitive and reproducible capillary electrophoresis method with UV detection for the separation and detection of cytosine and methylcytosine, after formic acid hydrolysis of DNA extracted from human whole blood. Hydrolysed samples were dried and resuspended with water and directly injected into the capillary without sample derivatization procedures. The use of a run buffer containing 50 mmol/L BIS-TRIS propane (BTP) phosphate buffer at pH 3.25 and 60 mmol/L sodium acetate buffer at pH 3.60 (4 : 1, v/v) allowed full analyte identification within 11 min. Precision tests indicated an elevated reproducibility with an interassay CV of 1.98% when starting from 2 μg of the extracted DNA. The method was successfully tested by measuring the DNA methylation degree both in healthy volunteers and in reference calf thymus DNA.
اللغةen
الناشرHindawi Publishing Corporation
الموضوعDNA Methylation
Capillary Electrophoresis
العنوانEvaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection
النوعArticle
رقم المجلد2017
ESSN2090-8873
dc.accessType Open Access


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