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المؤلفMarei, Hany El Sayed
المؤلفEl-Gamal, Aya
المؤلفAlthani, Asma
المؤلفAfifi, Nahla
المؤلفAbd-Elmaksoud, Ahmed
المؤلفFarag, Amany
المؤلفCenciarelli, Carlo
المؤلفThomas, Caceci
المؤلفAnwarul, Hasan
تاريخ الإتاحة2018-02-08T06:59:56Z
تاريخ النشر2018-02-01
اسم المنشورJournal of Cellular Physiology
المعرّفhttp://dx.doi.org/10.1002/jcp.25937
الاقتباسMarei HES, El-Gamal A, Althani A, et al. Cholinergic and dopaminergic neuronal differentiation of human adipose tissue derived mesenchymal stem cells. J Cell Physiol. 2018;233:936–945. https://doi.org/10.1002/jcp.25937
الرقم المعياري الدولي للكتاب0021-9541
معرّف المصادر الموحدhttp://hdl.handle.net/10576/6260
الملخصMesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types such as cartilage, bone, and fat cells. Recent studies have shown that induction of MSCs in vitro by growth factors including epidermal growth factor (EGF) and fibroblast growth factor (FGF2) causes them to differentiate into neural like cells. These cultures also express ChAT, a cholinergic marker; and TH, a dopaminergic marker for neural cells. To establish a protocol with maximum differentiation potential, we examined MSCs under three experimental culture conditions using neural induction media containing FGF2, EGF, BMP-9, retinoic acid, and heparin. Adipose-derived MSCs were extracted and expanded in vitro for 3 passages after reaching >80% confluency, for a total duration of 9 days. Cells were then characterized by flow cytometry for CD markers as CD44 positive and CD45 negative. MSCs were then treated with neural induction media and were characterized by morphological changes and Q-PCR. Differentiated MSCs expressed markers for immature and mature neurons; β Tubulin III (TUBB3) and MAP2, respectively, showing the neural potential of these cells to differentiate into functional neurons. Improved protocols for MSCs induction will facilitate and ensure the reproducibility and standard production of MSCs for therapeutic applications in neurodegenerative diseases.
اللغةen
الناشرWiley
الموضوعadipose tissue
cholinergic neurons
dopaminergic neurons
mesenchymal stem cell
العنوانCholinergic and dopaminergic neuronal differentiation of human adipose tissue derived mesenchymal stem cells.
النوعArticle
الصفحات12088-12088
رقم العدد7
رقم المجلد234
ESSN1097-4652
dc.accessType Abstract Only


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