A bioconjugated MoS2 based nanoplatform with increased binding efficiency to cancer cells
| Author | Kálosi, Anna |
| Author | Labudová, Martina |
| Author | Annušová, Adriana |
| Author | Benkovičová, Monika |
| Author | Bodík, Michal |
| Author | Kollár, Jozef |
| Author | Kotlár, Mário |
| Author | Kasak, Peter |
| Author | Jergel, Matej |
| Author | Pastoreková, Silvia |
| Author | Siffalovic, Peter |
| Author | Majkova, Eva |
| Available date | 2025-03-20T08:10:23Z |
| Publication Date | 2020 |
| Publication Name | Biomaterials Science |
| Resource | Scopus |
| Identifier | http://dx.doi.org/10.1039/c9bm01975h |
| ISSN | 20474830 |
| Abstract | We evaluate the application of surfactant-free liquid-phase exfoliated MoS2 nanosheets as a nanoplatform for a cancer detection and treatment system equipped with an antibody-antigen based recognition element. Employing antigen-antibody binding, we increased the probability of the endocytosis of MoS2 nanosheets into CAIX expressing cells by 30%. The nanosheets are functionalized with a specific antibody M75, which forms an antigen-antibody complex with CAIX. The bioconjugation of MoS2 nanosheets involves biocompatible components with low cytotoxicity, verified in the tested cell lines by fluorescence-based cell viability assay. The cellular internalization is quantified by flow cytometry, while the internalization is confirmed by label-free confocal Raman imaging. Raman measurements show increased lysosomal activity in the proximity of the internalized nanoplatforms. |
| Sponsor | This work was supported by the Slovak Research and Development Agency projects APVV-15-0641, APVV-16-0319, APVV-17-0352, APVV-17-0560, and APVV-18-0480, the Slovak Grant Agency for Science, VEGA 2/0059/19 and VEGA 2/0129/19, and the European Regional Development Fund (University Science Park for Biomedicine in Bratislava, ITMS26240220087). This study was performed during the implementation of the project Building-up Centre for advanced materials application of the Slovak Academy of Sciences, ITMS project code 313021T081 supported by the Research & Innovation Operational Programme funded by the ERDF. |
| Language | en |
| Publisher | Royal Society of Chemistry |
| Subject | MoS2 nanosheets cancer detection antigen-antibody binding cellular internalization Raman imaging |
| Type | Article |
| Pagination | 1973-1980 |
| Issue Number | 7 |
| Volume Number | 8 |
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