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AuthorAldisi, Rana S
AuthorElsidiq, Malaz S
AuthorDargham, Soha R
AuthorSahara, Afifah S
AuthorAl-Absi, Enas S
AuthorNofal, Mariam Y
AuthorMohammed, Layla I
AuthorAbu-Raddad, Laith J
AuthorNasrallah, Gheyath K
Available date2018-04-03T07:34:12Z
Publication Date2018-03-01
Publication NameJournal of Clinical Virology
Identifierhttp://dx.doi.org/10.1016/j.jcv.2018.03.011
CitationAldisi, Rana S. et al. Performance evaluation of four type-specific commercial assays for detection of herpes simplex virus type 1 antibodies in a Middle East and North Africa population. Journal of Clinical Virology , Volume 103 , pp. 1 - 7
ISSN1386-6532
URIhttp://hdl.handle.net/10576/6504
AbstractThe number of diagnostic assays for the detection of herpes simplex virus type 1 (HSV-1) antibodies has increased over the years. However, their performance characteristics could vary among global populations. To investigate performance of two commercial ELISA kits, HerpeSelect1 ELISA and Euroimmun Anti-HSV-1 (gC1) ELISA (IgG); and two commercial immunoblot (IB)/Western blot (WB) assays, HerpeSelect1 and 2 Immunoblot IgG, and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM); in detecting HSV-1 antibodies in a Middle East and North Africa (MENA) population. Blood specimens were collected from blood donors in Doha, Qatar, June 2013-2016. Twenty specimens were randomly selected from 10 MENA nationalities (Egypt, Iran, Jordan, Lebanon, Pakistan, Palestine, Qatar, Sudan, Syria, and Yemen; total = 200), and tested for HSV-1 antibodies. Across all six comparisons between assays, positive percent agreement ranged between 95.7% (95% CI: 91.4-98.3%) and 100.0% (95% CI: 97.8-100.0%). Negative percent agreement ranged between 86.2% (95% CI: 68.3-96.1%) and 96.2% (95% CI: 80.4-99.9%). Overall percent agreement ranged between 95.7% (95% CI: 91.7-97.8%) and 99.4% (95% CI: 96.7-99.9%). Cohen's kappa statistic ranged between 0.84 (95% CI: 0.73-0.95) and 0.98 (95% CI: 0.93-1.00). Compared against IB/WB, HerpeSelectand Euroimmun had sensitivities and specificities >96% and >86%, respectively. Positive and negative predictive values were >97% and >83%, respectively. The assays showed excellent concordance with one another, and with a high kappa statistic. The ELISA kits demonstrated robust diagnostic performance compared to the IB/WB assays. These findings support the assays' utility in clinical diagnosis and research in MENA populations.
Languageen
PublisherElsevier
SubjectCohen’s kappa statistic
Concordance measures
HSV-1
Sensitivity
Serology
Specificity
TitlePerformance evaluation of four type-specific commercial assays for detection of herpes simplex virus type 1 antibodies in a Middle East and North Africa population.
TypeArticle
Pagination1-7
Volume Number103 (2018)
ESSN1873-5967
dc.accessType Open Access


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