Performance evaluation of four type-specific commercial assays for detection of herpes simplex virus type 1 antibodies in a Middle East and North Africa population.
Author | Aldisi, Rana S |
Author | Elsidiq, Malaz S |
Author | Dargham, Soha R |
Author | Sahara, Afifah S |
Author | Al-Absi, Enas S |
Author | Nofal, Mariam Y |
Author | Mohammed, Layla I |
Author | Abu-Raddad, Laith J |
Author | Nasrallah, Gheyath K |
Available date | 2018-04-03T07:34:12Z |
Publication Date | 2018-03-01 |
Publication Name | Journal of Clinical Virology |
Identifier | http://dx.doi.org/10.1016/j.jcv.2018.03.011 |
Citation | Aldisi, Rana S. et al. Performance evaluation of four type-specific commercial assays for detection of herpes simplex virus type 1 antibodies in a Middle East and North Africa population. Journal of Clinical Virology , Volume 103 , pp. 1 - 7 |
ISSN | 1386-6532 |
Abstract | The number of diagnostic assays for the detection of herpes simplex virus type 1 (HSV-1) antibodies has increased over the years. However, their performance characteristics could vary among global populations. To investigate performance of two commercial ELISA kits, HerpeSelect1 ELISA and Euroimmun Anti-HSV-1 (gC1) ELISA (IgG); and two commercial immunoblot (IB)/Western blot (WB) assays, HerpeSelect1 and 2 Immunoblot IgG, and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM); in detecting HSV-1 antibodies in a Middle East and North Africa (MENA) population. Blood specimens were collected from blood donors in Doha, Qatar, June 2013-2016. Twenty specimens were randomly selected from 10 MENA nationalities (Egypt, Iran, Jordan, Lebanon, Pakistan, Palestine, Qatar, Sudan, Syria, and Yemen; total = 200), and tested for HSV-1 antibodies. Across all six comparisons between assays, positive percent agreement ranged between 95.7% (95% CI: 91.4-98.3%) and 100.0% (95% CI: 97.8-100.0%). Negative percent agreement ranged between 86.2% (95% CI: 68.3-96.1%) and 96.2% (95% CI: 80.4-99.9%). Overall percent agreement ranged between 95.7% (95% CI: 91.7-97.8%) and 99.4% (95% CI: 96.7-99.9%). Cohen's kappa statistic ranged between 0.84 (95% CI: 0.73-0.95) and 0.98 (95% CI: 0.93-1.00). Compared against IB/WB, HerpeSelectand Euroimmun had sensitivities and specificities >96% and >86%, respectively. Positive and negative predictive values were >97% and >83%, respectively. The assays showed excellent concordance with one another, and with a high kappa statistic. The ELISA kits demonstrated robust diagnostic performance compared to the IB/WB assays. These findings support the assays' utility in clinical diagnosis and research in MENA populations. |
Language | en |
Publisher | Elsevier |
Subject | Cohen’s kappa statistic Concordance measures HSV-1 Sensitivity Serology Specificity |
Type | Article |
Pagination | 1-7 |
Volume Number | 103 (2018) |
ESSN | 1873-5967 |
Check access options
Files in this item
This item appears in the following Collection(s)
-
Biomedical Sciences [692 items ]