Proteins of cottonseed (gossypium barbadense) .• extraction and characterization by electrophoresis

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Proteins of cottonseed (gossypium barbadense) .• extraction and characterization by electrophoresis

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dc.contributor.author Sammour, Reda Helmy [رضا حلمي سمور] en_US
dc.date.accessioned 2009-11-25T15:28:08Z
dc.date.available 2009-11-25T15:28:08Z
dc.date.issued 1995 en_US
dc.identifier.citation Qatar University Science Journal, 1995, Vol. 15, No. 1, Pages 77-82. en_US
dc.identifier.uri http://hdl.handle.net/10576/10143
dc.description.abstract The seed proteins of cotton (Gossypium barbadense) were quantitavely and qualitavely investigated. The qualitative study was carried out using different electrophoretic techniques ((PAGE, SDS-PAGE, Poro-PAGE, SDS-Poro-PAGE, 2-D SDS-PAGE, isoelectric focusing gels, and mapping gels). The quantities of the distilled water (albumin), salt (NaCl) (globulin), alcohol (prolamin) and alkaline solution (glu-telin) wer 126.4 +/- 1.51, 6.7.06 +/- 6.25, 30.78 +/- 4.2 and 79.40 +/- 3.1 mg/g dry meal respectively. Water soluble protein gave MW(s) of the reduced and unreduced buffer extracted proteins lies between 10 and 52 KD Second dimension gel showed that cottonseed proteins contain a disulphid bonded polypeptide with MW(s) 45 KD. Isoelectric points of the major polypeptides (the ploypeptides with MW(s) weights 52 KD and 48 KD) were between 9 and 9.5. The other polypeptides had pi-values between 5 and 7. Mapping gels, however indicated that the major bands were highly homogeneous with pi-values between 9-9.5. en_US
dc.description.abstract في هذا البحث درف البروتينات البذربة للقطن نوعيأ وكمياً . تم إجراء الدراسة النوعية بإستخد ام تقنيات الاستشراد . وقد أوضحت الدراسة الكمية للبروتينات المستخلصة . ar
dc.language.iso en en_US
dc.publisher Qatar University en_US
dc.subject Botany en_US
dc.subject علم النبات ar
dc.title Proteins of cottonseed (gossypium barbadense) .• extraction and characterization by electrophoresis en_US
dc.title.alternative البروتينات البذرية للقطن (GOSSYPIUM BARBADENSE) الفصل والتمييز باستخدام تقنيات الإستشراد ar
dc.type Article en_US
dc.identifier.pagination 77-82 en_US
dc.identifier.issue 1 en_US
dc.identifier.volume 15 en_US

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