Regeneration of Transformed Forage Legume Plants by Agrobacterium rhizogenes R1601

Abstract

Transformed forage legume plants (alfalfa & clover) by genetic engineering bacteria Agrobacterium rhizogenes R1601,were regenerated directly from seedling plants after inoculating the wounded site formed by cutting the plantlet stem at the cotyledon attachment site , and from hairy roots which formed on the hypocotyledon stem of these seedlings . Results show transgenic plants have large number of transformed shoots , with curled leaves compared to the control samples (seedlings plants),which were assayed by investigating the presence of the agropine using high voltage paper electrophoresis.

تم انتاج نباتات بقولية من النوع العكفي ( الجت والبرسيم ) محولة.