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Author Abdelkefi-Mesrati, Lobna
Author Boukedi, Hanen
Author Dammak-Karray, Mariam
Author Sellami-Boudawara, Tahya
Author Jaoua, Samir
Author Tounsi, Slim
Available date 2015-12-24T05:26:35Z
Publication Date 2011-02
Publication Name Journal of Invertebrate Pathologyen_US
Identifier http://dx.doi.org/10.1016/j.jip.2010.10.002
Citation "Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis", Abdelkefi-Mesrati, Lobna; Boukedi, Hanen; Dammak-Karray, Mariam; Sellami-Boudawara, Tahya; Jaoua, Samir et al, (2011) Journal of Invertebrate Pathology, vol. 106 (2) p. 250-254en_US
ISSN 0022-2011
ISSN 1096-0805 (online)
URI http://hdl.handle.net/10576/3981
Abstract The bacterium Bacillus thuringiensis produces, at the vegetative stage of its growth, Vip3A proteins with activity against a broad spectrum of lepidopteran insects. The Egyptian cotton leaf worm (Spodoptera littoralis) is an important agricultural pest that is susceptible to the Vip3Aa16 protein of Bacillus thuringiensis kurstaki strain BUPM95. The midgut histopathology of Vip3Aa fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formation in the apical region and cellular disintegration. Biotinylated Vip3Aa toxin bound proteins of 55- and 100-kDa on blots of S. littoralis brush border membrane preparations. These binding proteins differ in molecular size from those recognized by Cry1C, one of the very few Cry proteins active against the polyphagous S. littoralis. This result supports the use of Vip3Aa16 proteins as insecticidal agent, especially in case of Cry-resistance management.en_US
Sponsor Ministère de l’Enseignement Supérieur et de la Recherche Scientifiqueen_US
Language enen_US
Publisher Elsevier Inc.en_US
Subject Bacillus thuringiensisen_US
Subject Spodoptera littoralisen_US
Subject Vip3Aa16en_US
Subject Activationen_US
Subject Histopathological effecten_US
Subject Midgut putative receptorsen_US
Title Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralisen_US
Type Articleen_US
Pagination 250-254
Issue Number 2
Volume Number 106


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