Myocardial ischemic subject's thymus fat: A novel source of multipotent stromal cells
MetadataShow full item record
Objective Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells. Material and Methods We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects. Results TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs. Conclusions This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.
- Health Sciences-CAS (pre 2016) [123 items ]
Showing items related by title, author, creator and subject.
|Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers ||Roberts, Matthew J.; Richards, Renee S.; Chow, Clement W.K.; Doi, Suhail A.R.; Schirra, Horst Joachim; Buck, Marion; Samaratunga, Hemamali; Perry-Keene, Joanna; Payton, Diane; Yaxley, John; Lavin, Martin F.; Gardiner, Robert A.||2016||Elsevier||Article|
|Novel immunoassay technique for rapid measurement of intracellular proteins using paramagnetic particles ||Sharif, Elham; Kiely, Janice; Luxton, Richard||2013||Elsevier B.V.||Article|
|The Dual Role of Paramagnetic Particles for Integrated Lysis and Measurement in a Rapid Immunoassay for Intracellular Proteins ||Sharif, Elham; Kiely, Janice; Wraith, Patrick; Luxton, Richard||2013||IEEE||Article|