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AuthorGreenwood, Michael P.
AuthorMecawi, Andre S.
AuthorHoe, See Ziau
AuthorMustafa, Mohd Rais
AuthorJohnson, Kory R.
AuthorAl-Mahmoud, Ghada A.
AuthorElias, Lucila L. K.
AuthorPaton, Julian F. R.
AuthorAntunes-Rodrigues, Jose
AuthorGainer, Harold
AuthorMurphy, David
AuthorHindmarch, Charles C.T.
Available date2016-11-21T08:24:43Z
Publication Date2015-01-28
Publication NameAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology
Identifierhttp://dx.doi.org/10.1152/ajpregu.00444.2014
CitationGreenwood MP, Mecawi AS, Hoe SZ, et al. A comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. 2015;308(7):R559-R568.
ISSN0363-6119
URIhttp://hdl.handle.net/10576/5027
AbstractSalt loading (SL) and water deprivation (WD) are experimental challenges that are often used to study the osmotic circuitry of the brain. Central to this circuit is the supraoptic nucleus (SON) of the hypothalamus, which is responsible for the biosynthesis of the hormones, arginine vasopressin (AVP) and oxytocin (OXT), and their transport to terminals that reside in the posterior lobe of the pituitary. On osmotic challenge evoked by a change in blood volume or osmolality, the SON undergoes a function-related plasticity that creates an environment that allows for an appropriate hormone response. Here, we have described the impact of SL and WD compared with euhydrated (EU) controls in terms of drinking and eating behavior, body weight, and recorded physiological data including circulating hormone data and plasma and urine osmolality. We have also used microarrays to profile the transcriptome of the SON following SL and remined data from the SON that describes the transcriptome response to WD. From a list of 2,783 commonly regulated transcripts, we selected 20 genes for validation by qPCR. All of the 9 genes that have already been described as expressed or regulated in the SON by osmotic stimuli were confirmed in our models. Of the 11 novel genes, 5 were successfully validated while 6 were false discoveries.
SponsorThis work was supported by funding from the British Heart Foundation (RG/11/28714, to M. Greenwood, J. F. R. Paton, D. Murphy), the Biotechnology and Biological Sciences Research Council (BB/J005452/1, to C. C. T. Hindmarch, J. F. R. Paton, D. Murphy), and a High Impact Research Chancellory Grant (UM.C/625/1/HIR/MOHE/MED/22 H-20001-E000086) from the University of Malaya (to C. C. T. Hindmarch, S. Ziau Hoe, A. S. Mecawi, M. R. Mustafa, D. Murphy). This research was also supported by the Intramural Research Program of the National Institutes of Health, National Institute of Neurological Disorders and Stroke (for H. Gainer and K. R. Johnson).
Languageen
PublisherAmerican Physiological Society
Subjectneuroendocrine
salt load
supraoptic nucleus
Transcriptome
water restriction
TitleA comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus
TypeArticle
Issue Number7
Volume Number308
ESSN1522-1490
dc.accessType Abstract Only


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