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AuthorAl Disi, Zulfa
AuthorJaoua, Samir
AuthorAl-Thani, Dhabia
AuthorAl-Meer, Saeed
AuthorZouari, Nabil
Available date2017-02-19T10:19:58Z
Publication Date2017-01-24
Publication NameBioMed Research International
Identifierhttp://dx.doi.org/10.1155/2017/8649350
CitationZulfa Al Disi, Samir Jaoua, Dhabia Al-Thani, Saeed Al-Meer, and Nabil Zouari, “Considering the Specific Impact of Harsh Conditions and Oil Weathering on Diversity, Adaptation, and Activity of Hydrocarbon-Degrading Bacteria in Strategies of Bioremediation of Harsh Oily-Polluted Soils,” BioMed Research International, vol. 2017, Article ID 8649350, 11 pages, 2017
ISSN2314-6133
URIhttp://hdl.handle.net/10576/5280
AbstractWeathering processes change properties and composition of spilled oil, representing the main reason of failure of bioaugmentation strategies. Our purpose was to investigate the metabolic adaptation of hydrocarbon-degrading bacteria at harsh conditions to be considered to overcome the limitations of bioaugmentation strategies at harsh conditions. Polluted soils, exposed for prolonged periods to weathered oil in harsh soils and weather conditions, were used. Two types of enrichment cultures were employed using 5% and 10% oil or diesel as sole carbon sources with varying the mineral nitrogen sources and C/N ratios. The most effective isolates were obtained based on growth, tolerance to toxicity, and removal efficiency of diesel hydrocarbons. Activities of the newly isolated bacteria, in relation to the microenvironment from where they were isoalted and their interaction with the weathered oil, showed individual specific ability to adapt when exposed to such factors, to acquire metabolic potentialities. Among 39 isolates, ten identified ones by 16S rDNA genes similarities, including special two Pseudomonas isolates and one Citrobacter isolate, showed particularity of shifting hydrocarbon-degrading ability from short chain n-alkanes (n-C12–n-C16) to longer chain n-alkanes (n-C21–n-C25) and vice versa by alternating nitrogen source compositions and C/N ratios. This is shown for the first time.
SponsorThis work was supported by the College of Arts and Sciences and Department of Biological and Environmental Sciences of Qatar University [QUUG-CAS-DEM-12/13-6; QUST-CAS-FALL-12/13-23; and QUST-CAS-FALL-12/13-22].
Languageen
PublisherHindawi Publishing Corporation
SubjectOil Weathering on Diversity
Adaptation
Activity of Hydrocarbon-Degrading Bacteria
TitleConsidering the Specific Impact of Harsh Conditions and Oil Weathering on Diversity, Adaptation, and Activity of Hydrocarbon-Degrading Bacteria in Strategies of Bioremediation of Harsh Oily-Polluted Soils
TypeArticle
Volume Number2017
ESSN2314-6141
dc.accessType Open Access


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