Studies for determining antimicrobial activity of solenostemma argel (del) hayne. 2-extraction with chloroform/methanol in different proportions
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Aerial parts of Solenostemma argel plant were successively extracted with chloroform/methanol in different proportions (4 fractions). The phytochemical and chromatographic screening as well as quantitative determination of the flavonoid and saponin contents were carried out to each fraction. The antimicrobial activity of the four fractions against eight bacteria: Staphylococcus aureus; Micrococcus; Streptococcus spp; Bacillus anthracis; E. coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; and Proteus vulgaris and 14 fungi: Fusarium; Aspergillus parasiticus; A. flavus; A.jiiger; A. candidus; A. glaucus; Penicillum; Chrisosporium; Cr. neoformans; Candida spp; C. albicans; Can. spp 20; Mucor and Rhodotorula were studied. A powerful effect was observed in case of Streptococcus spp. and moderate action against E. coli. B. anthracis; S. aureus; Klebsiella pneumoniae and Proteus vulgaris. There was no effect on Micrococcus and Pseudomonas while a weak fungicidal activity was observed.