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AuthorAldubyan, Maha A
AuthorAlmami, Ibtesam S
AuthorBenslimane, Fatiha M
AuthorAlsonosi, Abdlrhman M
AuthorForsythe, Stephen J
Available date2018-12-19T04:53:26Z
Publication Date2017-11-17
Publication NameFrontiers in Microbiologyen_US
Identifierhttp://dx.doi.org/10.3389/fmicb.2017.02268
CitationAldubyan MA, Almami IS, Benslimane FM, Alsonosi AM and Forsythe SJ (2017) Comparative Outer Membrane Protein Analysis of High and Low-Invasive Strains of Cronobacter malonaticus. Front. Microbiol. 8:2268. doi: 10.3389/fmicb.2017.02268
URIhttp://hdl.handle.net/10576/11209
Abstractare an important group of foodborne pathogens that has been linked to life-threatening infections in both infants and adults. The major infections associated with species are neonatal meningitis, necrotizing enterocolitis, and septicaemia. There are seven species in the genus, of which only three are of clinical importance; , and . To date most studies have focussed on as it is the major species associated with neonatal infections. However, recently , in particular sequence type 7 (ST7), has been noted as being prevalent in adult infections and therefore warranting further investigation. In this study, eight strains of ST7, that had been isolated from a wide range of sources and varied in their virulence, were chosen for proteomic analysis of their outer membrane proteins (OMPs). One-dimensional gel analysis revealed a ~29 kDa size band that was only present in the highly invasive strains. Subsequent mass spectrometric analysis identified several peptides that matched the flagellin protein. The presence of flagellin protein was confirmed in 2D gel spot. Mass spectrometry analysis of total OMPs revealed that the four highly invasive strains expressed the main flagellum proteins that were absent from the four low invasive strains. These were the flagellar hook protein FlgE, flagellar hook-associated protein 1, flagellar hook-associated protein, flagellin, and flagellar hook-filament junction protein FlgL. This data indicates that flagellar proteins may have an important role in the organism's invasion properties.
Languageen
PublisherFrontiers Media
SubjectCronobacter malonaticus
SubjectCronobacter spp.
Subjectflagellin
Subjectinvasive
Subjectouter membrane proteins (OMPs)
Subjectproteomics
TitleComparative Outer Membrane Protein Analysis of High and Low-Invasive Strains of .
TypeArticle
Pagination1-11
Volume Number8
dc.identifier.essn 1664-302X


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