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المؤلفKahbasi S.
المؤلفSamadbin M.
المؤلفAttar F.
المؤلفHeshmati M.
المؤلفDanaei D.
المؤلفRasti B.
المؤلفSalihi A.
المؤلفNanakali N.M.Q.
المؤلفAziz F.M.
المؤلفAkhtari K.
المؤلفHasan A.
المؤلفFalahati M.
تاريخ الإتاحة2020-04-15T12:01:42Z
تاريخ النشر2019
اسم المنشورInternational Journal of Biological Macromolecules
المصدرScopus
الرقم المعياري الدولي للكتاب1418130
معرّف المصادر الموحدhttp://dx.doi.org/10.1016/j.ijbiomac.2019.07.154
معرّف المصادر الموحدhttp://hdl.handle.net/10576/14174
الملخصHerein, we explored the interaction of Al2O3 NPs with RBCs and Hb to determine the effect of Al2O3 NPs on hemolytic activity and Hb denaturation. The percentage of hemolysis of extracts and direct contact assays triggered by Al2O3 NPs was calculated by determining supernatant Hb concentration at 540 nm. Far-UV CD and Trp/ANS/acrylamide fluorescence spectroscopic methods were used to determine the structural changes of Hb upon interaction with Al2O3 NPs. Theoretical studies were carried out to display the residues involved in the binding site of Hb with Al2O3 nanocluster as well as the structural changes of Hb after interaction. The results showed that the percentage of hemolysis of extract and direct contact assays induced by Al2O3 NPs were 1.16 and 0.46, respectively. Fluorescence spectroscopy revealed that Al2O3 NPs alter the quaternary structure of the protein; however, CD spectroscopy indicated that the secondary structure of Hb remains almost unchanged. Theoretical study displayed that Al2O3 nanocluster interacts with different residues of protein, and Hb tends to be destabilized at the binding site with nanocluster. This study may be significant in exploring the toxicity profile of Al2O3 NPs for their in vivo implementations. - 2019
راعي المشروعThis research was made possible by the grants NPRP10-120-170-211 from Qatar National Research Fund (QNRF) under Qatar Foundation and GCC-2017-005 under the GCC collaborative research Program from Qatar University . The statements made herein are the sole responsibility of the authors.
اللغةen
الناشرElsevier B.V.
الموضوعAluminum oxide
Hemoglobin structure
Hemolysis
Nanoparticles
Spectroscopy
Theoretical studies
Toxicity
العنوانThe effect of aluminum oxide on red blood cell integrity and hemoglobin structure at nanoscale
النوعArticle
الصفحات800-809
رقم المجلد138
dc.accessType Abstract Only


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