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AuthorKahbasi S.
AuthorSamadbin M.
AuthorAttar F.
AuthorHeshmati M.
AuthorDanaei D.
AuthorRasti B.
AuthorSalihi A.
AuthorNanakali N.M.Q.
AuthorAziz F.M.
AuthorAkhtari K.
AuthorHasan A.
AuthorFalahati M.
Available date2020-04-15T12:01:42Z
Publication Date2019
Publication NameInternational Journal of Biological Macromolecules
ResourceScopus
ISSN1418130
URIhttp://dx.doi.org/10.1016/j.ijbiomac.2019.07.154
URIhttp://hdl.handle.net/10576/14174
AbstractHerein, we explored the interaction of Al2O3 NPs with RBCs and Hb to determine the effect of Al2O3 NPs on hemolytic activity and Hb denaturation. The percentage of hemolysis of extracts and direct contact assays triggered by Al2O3 NPs was calculated by determining supernatant Hb concentration at 540 nm. Far-UV CD and Trp/ANS/acrylamide fluorescence spectroscopic methods were used to determine the structural changes of Hb upon interaction with Al2O3 NPs. Theoretical studies were carried out to display the residues involved in the binding site of Hb with Al2O3 nanocluster as well as the structural changes of Hb after interaction. The results showed that the percentage of hemolysis of extract and direct contact assays induced by Al2O3 NPs were 1.16 and 0.46, respectively. Fluorescence spectroscopy revealed that Al2O3 NPs alter the quaternary structure of the protein; however, CD spectroscopy indicated that the secondary structure of Hb remains almost unchanged. Theoretical study displayed that Al2O3 nanocluster interacts with different residues of protein, and Hb tends to be destabilized at the binding site with nanocluster. This study may be significant in exploring the toxicity profile of Al2O3 NPs for their in vivo implementations. - 2019
SponsorThis research was made possible by the grants NPRP10-120-170-211 from Qatar National Research Fund (QNRF) under Qatar Foundation and GCC-2017-005 under the GCC collaborative research Program from Qatar University . The statements made herein are the sole responsibility of the authors.
Languageen
PublisherElsevier B.V.
SubjectAluminum oxide
Hemoglobin structure
Hemolysis
Nanoparticles
Spectroscopy
Theoretical studies
Toxicity
TitleThe effect of aluminum oxide on red blood cell integrity and hemoglobin structure at nanoscale
TypeArticle
Pagination800-809
Volume Number138
dc.accessType Abstract Only


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