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المؤلفZribi Zghal R.
المؤلفFrikha F.
المؤلفElleuch J.
المؤلفDarriet F.
المؤلفChandre F.
المؤلفJaoua S.
المؤلفTounsi S.
تاريخ الإتاحة2020-04-25T01:02:20Z
تاريخ النشر2019
اسم المنشورInternational Journal of Biological Macromolecules
المصدرScopus
الرقم المعياري الدولي للكتاب1418130
معرّف المصادر الموحدhttp://dx.doi.org/10.1016/j.ijbiomac.2018.10.226
معرّف المصادر الموحدhttp://hdl.handle.net/10576/14438
الملخصCyt1A98 is a novel cytolytic protein, from BUPM98 Bacillus thuringiensis strain, characterized by its synergistic activity with B. thuringiensis kurstaki toxins against lepidopteran larvae. In this study, we evidenced that Cyt1A98 improves the toxicity of B. thuringiensis kurstaki toxins against Aedes aegypti larvae. In fact, the strain BNS3pHTcyt1A98 exhibited a larvicidal activity of about 849-fold of that of BNS3pHTBlue against A. aegypti. The molecular and biochemical characterizations, of cyt1A98 gene and its product, were achieved. Cyt1A98 had an LC50 value of about 126.56 mg l−1 against A. aegypti larvae. Compared to Cyt1Aa of B. thuringiensis israelensis, Cyt1A98 amino acid sequence harbours three substitutions of three conserved amino acids among Cyt1Aa family members (Ser42Pro, Pro82Ala, Met188Thr). The Cyt1A98 protein structural analysis evidenced more flexibility than Cyt1Aa. According to the high fluctuation observed for the residue Pro42, the amino acid at position 42 is implicated in the flexibility property of Cyt1Aa especially for the αC and αD helices, involved in the penetration into the cell membrane. The toxicity improvement could be probably due to the higher flexibility combined with the specific affinity toward dipteran larvae. The Cyt1A/B. thuringiensis kurstaki Cry toxins model provides a potential molecular genetic strategy for an efficient bioinsecticide.
راعي المشروعThis work was supported by grants from the Tunisian Ministry of Higher Education and Scientific Research . The authors wish to thank Mr. Abdelmajid Dammak, English professor at the National School of Engineers of Sfax, Tunisia, for his proof reading of the manuscript.
اللغةen
الناشرElsevier B.V.
الموضوعCyt1A98 expression
Cyt1Aa flexibility
Larvicidal activity improvement
العنوانThe combinatory effect of Cyt1Aa flexibility and specificity against dipteran larvae improves the toxicity of Bacillus thuringensis kurstaki toxins
النوعArticle
الصفحات42-49
رقم المجلد123
dc.accessType Abstract Only


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