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AuthorCheng X.
AuthorHuang L.
AuthorYang X.
AuthorElzatahry A.A.
AuthorAlghamdi A.
AuthorDeng Y.
Available date2020-04-25T01:02:22Z
Publication Date2019
Publication NameJournal of Colloid and Interface Science
AbstractOwing to the high costs and instability of natural enzymes, the development of enzyme mimics based on inorganic nanomaterials has attracted a wide concern in recent years. In this work, a stable nanocomposite composed of highly dispersed CeO2 nanoparticles decorated on zeolite Y as support (CeO2/Y) was synthesized by a facile wet impregnation method, and the CeO2/Y nanocomposite was firstly proposed as an efficient peroxidase-mimicking nanozyme for accurate detection of H2O2 and glucose. The best catalyst was the nanocomposite with CeO2 to zeolite Y mass ratio of 0.20 (denoted as 20CeO2/Y), showing a better affinity and higher catalytic constant to the substrate of H2O2 and 3,3′,5,5′-tetramethylbenzidine (TMB) than horseradish peroxidase (HRP) enzymes by the steady-state kinetic analysis. The enzyme-mimicking catalyst could be used over a wider range of pH and temperature for a long-time reuse in TMB oxidation. A facile colorimetric assay was set up for the accurate detection of H2O2 and glucose, with the detection limits of 0.323 μM and 35.4 μM, respectively. The CeO2/Y-based peroxidase mimic was used to precisely detect the glucose concentration in real blood serum samples, exhibiting a great potential to be constructed as a reliable biosensor for glucose detection in some complex systems. The superior peroxidase-mimicking performance of CeO2/Y nanocomposite is attributed to the synergistic effects of outstanding activity of highly dispersed CeO2 nanoparticles (5–10 nm) and adsorption properties of zeolite Y with large surface area (517 m2·g−1) and pore volume (0.329 cm3·g−1).
SponsorThis work was sponsored by Shanghai Pujiang Program , China (No. 16PJ1401100 ), and the Shanghai Committee of Science and Technology , China (No. 15ZR1402000 ), Key Basic Research Program of Science and Technology Commission of Shanghai Municipality ( 17JC1400100 ), the NSF of China (51372041 , 51422202 , 21673048 , and 21875044 ), Youth Top-notch Talent Support Program of China.
PublisherAcademic Press Inc.
SubjectCeO2/Y nanocomposite
SubjectGlucose detection
SubjectPeroxidase mimic
TitleRational design of a stable peroxidase mimic for colorimetric detection of H2O2 and glucose: A synergistic CeO2/Zeolite Y nanocomposite
Volume Number535

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