Characterization of Cellobiohydrolases from Schizophyllum commune KMJ820
| Author | Kondaveeti, Sanath |
| Author | Patel, Sanjay K. S. |
| Author | Woo, Janghun |
| Author | Wee, Ji Hyang |
| Author | Kim, Sang-Yong |
| Author | Al-Raoush, Riyadh I. |
| Author | Kim, In-Won |
| Author | Kalia, Vipin Chandra |
| Author | Lee, Jung-Kul |
| Available date | 2020-08-18T08:34:15Z |
| Publication Date | 2019 |
| Publication Name | Indian Journal of Microbiology |
| Resource | Scopus |
| ISSN | 468991 |
| Abstract | A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51�U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (Vmax = 51.4 and 20.8�U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50�kDa and 150�kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity. - 2019, Association of Microbiologists of India. |
| Sponsor | This research was supported by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2019R1C1C1009766, NRF-2019H1D3A2A01060226, 2019R1F1A1063131). This research was supported by 2018 KU Brain Pool fellowship of Konkuk University. |
| Language | en |
| Publisher | Springer |
| Subject | Catalytic efficiency Cellobiohydrolase Enzyme production Enzyme purification Schizophyllum commune |
| Type | Article |
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Civil and Environmental Engineering [858 items ]