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AuthorMasmoudi, Fatma
AuthorKhedher, Saoussen Ben
AuthorKamoun, Amel
AuthorZouari, Nabil
AuthorTounsi, Slim
AuthorTrigui, Mohamed
Available date2020-11-04T10:00:40Z
Publication Date2017
Publication NameMicrobiological Research
ResourceScopus
URIhttp://dx.doi.org/10.1016/j.micres.2017.01.001
URIhttp://hdl.handle.net/10576/16901
AbstractThis work is directed towards Bacillus amyloliquefaciens strain BLB371 metabolite production for biocontrol of fungal phytopathogens. In order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. After three rounds of mutagenesis, a hyper active mutant, named M3-7, was obtained. It produces 7 fold more antifungal metabolites (1800 AU/mL) than the wild strain in MC medium. A hybrid design was applied to optimise a new medium to enhance antifungal metabolite production by M3-7. The new optimized medium (35 g/L of peptone, 32.5 g/L of sucrose, 10.5 g/L of yeast extract, 2.4 g/L of KH2PO4, 1.3 g/L of MgSO4 and 23 mg/L of MnSO4) achieved 1.62 fold enhancement in antifungal compound production (3000 AU/mL) by this mutant, compared to that achieved in MC medium. Therefore, combinatory effect of these two approaches (mutagenesis and medium component optimization) allowed 12 fold improvement in antifungal activity (from 250 UA/mL to 3000 UA/mL). This improvement was confirmed against several phytopathogenic fungi with an increase of MIC and MFC over than 50%. More interestingly, a total eradication of gray mold was obtained on tomato fruits infected by Botrytis cinerea and treated by M3-7, compared to those treated by BLB371. From the practical point of view, combining random mutagenesis and medium optimization could be considered as an excellent tool for obtaining promising biological products useful against phytopathogenic fungi.
Languageen
PublisherElsevier GmbH
SubjectAntifungal metabolites
Bacillus amyloliquefaciens
Biological control
Hybrid design
Medium optimization
Mutagenesis
TitleCombinatorial effect of mutagenesis and medium component optimization on Bacillus amyloliquefaciens antifungal activity and efficacy in eradicating Botrytis cinerea
TypeArticle
Pagination29-38
Volume Number197
dc.accessType Abstract Only


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