Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable Disease Severities.
المؤلف | Al Khatib, Hebah A |
المؤلف | Benslimane, Fatiha M |
المؤلف | Elbashir, Israa E |
المؤلف | Coyle, Peter V |
المؤلف | Al Maslamani, Muna A |
المؤلف | Al-Khal, Abdullatif |
المؤلف | Al Thani, Asmaa A |
المؤلف | Yassine, Hadi M |
تاريخ الإتاحة | 2020-11-09T07:21:54Z |
تاريخ النشر | 2020-10-06 |
اسم المنشور | Frontiers in Cellular and Infection Microbiology |
المعرّف | http://dx.doi.org/10.3389/fcimb.2020.575613 |
الاقتباس | Al Khatib HA, Benslimane FM, Elbashir IE, Coyle PV, Al Maslamani MA, Al-Khal A, Al Thani AA and Yassine HM (2020) Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable Disease Severities. Front. Cell. Infect. Microbiol. 10:575613. doi: 10.3389/fcimb.2020.575613 |
الملخص | The ongoing pandemic of SARS-COV-2 has already infected more than eight million people worldwide. The majority of COVID-19 patients either are asymptomatic or have mild symptoms. Yet, about 15% of the cases experience severe complications and require intensive care. Factors determining disease severity are not yet fully characterized. Here, we investigated the within-host virus diversity in COVID-19 patients with different clinical manifestations. We compared SARS-COV-2 genetic diversity in 19 mild and 27 severe cases. Viral RNA was extracted from nasopharyngeal samples and sequenced using the Illumina MiSeq platform. This was followed by deep-sequencing analyses of SARS-CoV-2 genomes at both consensus and sub-consensus sequence levels. Consensus sequences of all viruses were very similar, showing more than 99.8% sequence identity regardless of the disease severity. However, the sub-consensus analysis revealed significant differences in within-host diversity between mild and severe cases. Patients with severe symptoms exhibited a significantly (-value 0.001) higher number of variants in coding and non-coding regions compared to mild cases. Analysis also revealed higher prevalence of some variants among severe cases. Most importantly, severe cases exhibited significantly higher within-host diversity (mean = 13) compared to mild cases (mean = 6). Further, higher within-host diversity was observed in patients above the age of 60 compared to the younger age group. These observations provided evidence that within-host diversity might play a role in the development of severe disease outcomes in COVID-19 patients; however, further investigations are required to elucidate this association. |
راعي المشروع | This work was supported by Qatar University under internal grant (QUCG-BRC-20/21-1) and Qatar National Research Fund grant under grant (RRC-2-039). |
اللغة | en |
الناشر | Frontiers Media |
الموضوع | COVID-19 severity SARS-CoV-2 nonsynonymous mutations virus quasispecies within-host diversity |
النوع | Article |
الصفحات | 534 |
رقم المجلد | 10 |
ESSN | 2235-2988 |
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