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AuthorShurbaji, Samar
AuthorEl-Sherbiny, Ibrahim M
AuthorAlser, Maha
AuthorAli, Isra H
AuthorKordi, Haya
AuthorAl-Sadi, Ameena
AuthorPopelka, Anton
AuthorBenslimane, Fatiha
AuthorYacoub, Magdi
AuthorYalcin, Huseyin C
Available date2021-01-31T05:56:51Z
Publication Date2021-01-05
Publication NameFrontiers in Bioengineering and Biotechnology
Identifierhttp://dx.doi.org/10.3389/fbioe.2020.579788
CitationShurbaji S, El-Sherbiny IM, Alser M, Ali IH, Kordi H, Al-Sadi A, Popelka A, Benslimane F, Yacoub M and Yalcin HC (2021) Nitric Oxide Releasing Hydrogel Nanoparticles Decreases Epithelial Cell Injuries Associated With Airway Reopening. Front. Bioeng. Biotechnol. 8:579788. doi: 10.3389/fbioe.2020.579788
URIhttp://hdl.handle.net/10576/17539
AbstractAcute respiratory distress syndrome (ARDS) is an acute inflammatory lung condition. It is characterized by disruption of gas exchange inside the alveoli, accumulation of protein edema, and an increase in lung stiffness. One major cause of ARDS is a lung infection, such as SARS-COV-2 infection. Lungs of ARDS patients need to be mechanically ventilated for airway reopening. Consequently, ventilation might damage delicate lung tissue leading to excess edema, known as ventilator-induced lung injury (VILI). Mortality of COVID-19 patients under VILI seems to be higher than non-COVID patients, necessitating effective preventative therapies. VILI occurs when small air bubbles form in the alveoli, injuring epithelial cells (EPC) due to shear stress. Nitric oxide (NO) inhalation was suggested as a therapy for ARDS, however, it was shown that it is not effective because of the extremely short half-life of NO. In this study, NO-releasing nanoparticles were produced and tested in an model, representing airways in the deep lung. Cellular injuries were quantified via fluorescent live/dead assay. Atomic force microscopy (AFM) was used to assess cell morphology. qRT-PCR was performed to assess the expression of inflammatory markers, specifically IL6 and CCL2. ELISA was performed to assess IL6 and confirm qRT-PCR results at the protein level. Finally, ROS levels were assessed in all groups. Here, we show that NO delivery via nanoparticles enhanced EPC survival and recovery, AFM measurements revealed that NO exposure affect cell morphology, while qRT-PCR demonstrated a significant downregulation in IL6 and CCL2 expression when treating the cells to NO both before and after shear exposure. ELISA results for IL6 confirmed qRT-PCR data. ROS experiment results support our findings from previous experiments. These findings demonstrate that NO-releasing nanoparticles can be used as an effective delivery approach of NO to deep lung to prevent/reduce ARDS associated inflammation and cell injuries. This information is particularly useful to treat severe ARDS due to COVID-19 infection. These nanoparticles will be useful when clinically administrated to COVID-19 patients to reduce the symptoms originating from lung distress.
Languageen
PublisherFrontiers
SubjectCOVID-19
SARS-CoV-2
acute respiratory distress syndrome
hydrogel nanoparticle
mechanical ventilation
nitric oxide
TitleNitric Oxide Releasing Hydrogel Nanoparticles Decreases Epithelial Cell Injuries Associated With Airway Reopening.
TypeArticle
Volume Number8
dc.accessType Open Access


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