Evaluation of antibody response in symptomatic and asymptomatic covid-19 patients and diagnostic assessment of new IgM/IgG elisa kits
Author | Al-Jighefee, Hadeel T. |
Author | Yassine, Hadi M. |
Author | Nasrallah, Gheyath K. |
Available date | 2021-02-23T06:20:58Z |
Publication Date | 2021-02-01 |
Publication Name | Pathogens |
Identifier | http://dx.doi.org/10.3390/pathogens10020161 |
Citation | Al-Jighefee, H.T.; Yassine, H.M.; Al-Nesf, M.A.; Hssain, A.A.; Taleb, S.; Mohamed, A.S.; Maatoug, H.; Mohamedali, M.; Nasrallah, G.K. Evaluation of Antibody Response in Symptomatic and Asymptomatic COVID-19 Patients and Diagnostic Assessment of New IgM/IgG ELISA Kits. Pathogens 2021, 10, 161. https://doi.org/10.3390/pathogens10020161 |
Abstract | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This study aims to study the immune response and evaluate the performances of four new IgM and five IgG enzyme-linked immunosorbent assay (ELISA) kits for detecting anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies against different antigens in symptomatic and asymptomatic coronavirus disease 2019 (COVID-19) patients. A total of 291 samples collected from symptomatic and asymptomatic RT–PCR-confirmed patients were used to evaluate the ELISA kits’ performance (EDI, AnshLabs, DiaPro, NovaLisa, and Lionex). The sensitivity was measured at three different time-intervals post symptoms onset or positive SARS-CoV-2 RT–PCR test (≤14, 14–30, >30 days). The specificity was investigated using 119 pre-pandemic serum samples. The sensitivity of all IgM kits gradually decreased with time, ranging from 48.7% (EDI)–66.4% (Lionex) at ≤14 days, 29.1% (NovaLisa)–61.8% (Lionex) at 14–30 days, and 6.0% (AnshLabs)–47.9% (Lionex) at >30 days. The sensitivity of IgG kits increased with time, peaking in the latest interval (>30 days) at 96.6% (Lionex). Specificity of IgM ranged from 88.2% (Lionex)–99.2% (EDI), while IgG ranged from 75.6% (DiaPro)–98.3% (Lionex). Among all RT–PCR-positive patients, 23 samples (7.9%) were seronegative by all IgG kits, of which only seven samples (30.4%) had detectable IgM antibodies. IgM assays have variable and low sensitivity, thus considered a poor marker for COVID-19 diagnosis. IgG assays can miss at least 8% of RT–PCR-positive cases. |
Language | en |
Publisher | MDPI |
Subject | Asymptomatic COVID-19 ELISA IgG IgM SARS-CoV-2 Sensitivity Serology Specificity Symptomatic |
Type | Article |
Issue Number | 2 |
Volume Number | 10 |
ESSN | 2076-0817 |
Files in this item
This item appears in the following Collection(s)
-
Biomedical Research Center Research [727 items ]
-
COVID-19 Research [811 items ]