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AuthorAlmajali, Belal
AuthorAl-Jamal, Hamid Ali Nagi
AuthorWan Taib, Wan Rohani
AuthorIsmail, Imilia
AuthorJohan, Muhammad Farid
AuthorDoolaanea, Abd Almonem
AuthorIbrahim, Wisam Nabeel
AuthorTajudin, Syed Ahmad
Available date2021-03-30T08:57:31Z
Publication Date2021-03-01
Publication NameAsian Pacific Journal of Cancer Prevention (APJCP)en_US
Identifierhttp://dx.doi.org/10.31557/APJCP.2021.22.3.879
ISSN1513-7368
URIhttp://hdl.handle.net/10576/18039
AbstractThe natural compound, thymoquinone (TQ) has demonstrated potential anticancer properties in inhibiting cell proliferation and promoting apoptosis in myeloid leukemia cells, breast cancer cells, and others. However, the effect mechanism of TQ on AML cells still not fully understood. In this study, the authors examined the effects of TQ on the expression of JAK/STAT-negative regulator genes SOCS-1, SOCS-3, and SHP-1, and their consequences on cell proliferation and apoptosis in HL60 leukemia cells. MTT and trypan blue exclusion tests were conducted to determine the 50% inhibitory concentration (IC50) and cell proliferation. FITC Annexin and Guava® reagent were used to study the cell apoptosis and examine the cell cycle phases, respectively. The expression of JAK/STAT-negative regulator genes, SOCS-1, SOCS-3, and SHP-1, was investigated using reverse transcriptase- quantitative PCR (RT-qPCR). TQ demonstrated a potential inhibition of HL60 cell proliferation and a significant increase in apoptotic cells in dose and time-dependent manner. TQ significantly induced cycle arrest at G0-G1 phase (P < 0.001) and enhanced the re-expression of JAK/STAT-negative regulator genes. TQ potentially inhibited HL60 cell proliferation and significantly increased apoptosis with re-expression of JAK/STAT-negative regulator genes suggesting that TQ could be a new therapeutic candidate for leukemia therapy.<br />.
SponsorThis work was funded by the Fundamental Research Grant Scheme of the Ministry of Education, Malaysia [FRGS/1/2019/SKK08/UNISZA/02/3 (RR330)].
Languageen
PublisherWest Asia Organization for Cancer Prevention (WAOCP)
SubjectJAK/STAT signaling
SubjectLeukemia
Subjectnegative regulators
Subjectthymoquinone
TitleThymoquinone Suppresses Cell Proliferation and Enhances Apoptosis of HL60 Leukemia Cells through Re-Expression of JAK/STAT Negative Regulators.
TypeArticle
Pagination879-885
Issue Number3
Volume Number22
ESSN2476-762X


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